RAVER1 Antibody - BSA Free

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Immunohistochemistry: RAVER1 Antibody [NBP2-47313] - Immunohistochemistry analysis in human small intestine and skeletal muscle tissues using NBP2-47313 antibody. Corresponding RAVER1 RNA-seq data are presented for the ...read more
Orthogonal Strategies: Western Blot:RAVER1 Antibody:NBP2-47313-Analysis in human cell line HEK 293 and human cell line SK-MEL-30.
Immunohistochemistry: RAVER1 Antibody [NBP2-47313] - Staining of human skeletal muscle shows low positivity in myocytes as expected.
Immunohistochemistry: RAVER1 Antibody [NBP2-47313] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry: RAVER1 Antibody [NBP2-47313] - Staining of human small intestine shows strong nuclear positivity in glandular cells.
Immunohistochemistry: RAVER1 Antibody [NBP2-47313] - Staining of human lymphoid tissues shows strong nuclear positivity in non-germinal center cells.
Staining of human cell line A-431 shows localization to nucleoplasm.
Independent Antibodies: Analysis using NBP2-47313 (A) shows similar pattern to independent antibody NBP2-48795 (B).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

RAVER1 Antibody - BSA Free Summary

Description
Novus Biologicals Rabbit RAVER1 Antibody - BSA Free (NBP2-47313) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LLPEPNILQLLNNLGPSASLQLLLNPLLHGSAGGKQGLLGAPPAMPLLNGPALSTALLQLALQTQGQKKPGILGDSPLG
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAVER1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 µg/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RAVER1 Protein (NBP2-47313PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for RAVER1 Antibody - BSA Free

  • KIAA1978Protein raver-1
  • ribonucleoprotein PTB-binding 1
  • ribonucleoprotein, PTB-binding 1

Background

Cooperates with PTBP1 to modulate regulated alternative splicing events. Promotes exon skipping. Cooperateswith PTBP1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA (Bysimilarity)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RAVER1