Rat CXCL1/CINC-1 DuoSet ELISA, 15 Plate

• Reactivity: Rt
• Applications: ELISA
• Conjugate: Biotin

Rat CXCL1/CINC-1 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Cxcl1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat CXCL1/CINC-1 DuoSet ELISA, 15 Plate

• CINC1
• CINC-1
• CXCL1
• FSP
• GRO alpha
• GRO1
• GROa
• KC
• MGSA
• MGSA-a
• MGSA-alpha
• NAP-3
• SCYB1

Background

CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.

Publications for CXCL1/GRO alpha/KC/CINC-1 (DY515)(18)

Publications using DY515

• Tojo, K;Yazawa, T; A high fraction of inspired oxygen does not mitigate atelectasis-induced lung tissue hypoxia or injury in experimental acute respiratory distress syndrome Scientific reports 2025-01-14 [PMID: 39809828] (Rat)
• Kuo, CH;Lee, GH;Wu, HL;Huang, JY;Tang, MJ; Breaking the symmetry of cell contractility drives tubulogenesis via CXCL1 polarization Proceedings of the National Academy of Sciences of the United States of America 2024-02-27 [PMID: 38377213] (Rat)
• NA Lage, MRB de Paiva, DV Vasconcelo, RR Machado, SL Fialho, A Silva-Cunh Efficacy and Safety Evaluation of Mometasone Furoate in Treating Ocular Inflammation Pharmaceutics, 2023-01-05;15(1):. 2023-01-05 [PMID: 36678822] (Rat)
• N Shologu, M Scully, JG Laffey, D O'Toole Human Mesenchymal Stem Cell Secretome from Bone Marrow or Adipose-Derived Tissue Sources for Treatment of Hypoxia-Induced Pulmonary Epithelial Injury Int J Mol Sci, 2018-09-30;19(10):. 2018-09-30 [PMID: 30274394] (Human)
• C Francioli, X Wang, R Parapanov, E Abdelnour, J Lugrin, F Gronchi, J Perentes, P Eckert, HB Ris, L Piquilloud, T Krueger, L Liaudet Pyrrolidine dithiocarbamate administered during ex-vivo lung perfusion promotes rehabilitation of injured donor rat lungs obtained after prolonged warm ischemia PLoS ONE, 2017-03-21;12(3):e0173916. 2017-03-21 [PMID: 28323904] (Rat)
• S Bettini, E Boutet-Rob, C Cartier, C Com‚ra, E Gaultier, J Dupuy, N Naud, S Tach‚, P Grysan, S Reguer, N Thieriet, M R‚fr‚giers, D ThiaudiŠre, JP Cravedi, M CarriŠre, JN Audinot, FH Pierre, L Guzylack-P, E Houdeau Food-grade TiO2 impairs intestinal and systemic immune homeostasis, initiates preneoplastic lesions and promotes aberrant crypt development in the rat colon Sci Rep, 2017-01-20;7(0):40373. 2017-01-20 [PMID: 28106049] (Rat)
• Cláudia Herrera Tambeli Intra-articular blockade of P2X7 receptor reduces the articular hyperalgesia and inflammation in the knee joint synovitis especially in female rats J Pain, 2016-11-04;0(0):. 2016-11-04 [PMID: 27818192] (Rat)
• Summers L, Kangwantas K, Rodriguez-Grande B, Denes A, Penny J, Kielty C, Pinteaux E Activation of brain endothelial cells by interleukin-1 is regulated by the extracellular matrix after acute brain injury. Mol Cell Neurosci, 2013-10-24;57(0):93-103. 2013-10-24 [PMID: 24161715] (Rat)
• Roddy GW, Oh JY, Lee RH, Bartosh TJ, Ylostalo J, Coble K, Rosa RH, Prockop DJ Action at a distance: systemically administered adult stem/progenitor cells (MSCs) reduce inflammatory damage to the cornea without engraftment and primarily by secretion of TNF-alpha stimulated gene/protein 6. Stem Cells, 2011-10-01;29(10):1572-9. 2011-10-01 [PMID: 21837654] (Rat)
• Barichello T, dos Santos I, Savi GD, Simoes LR, Silvestre T, Comim CM, Sachs D, Teixeira MM, Teixeira AL, Quevedo J TNF-alpha, IL-1beta, IL-6, and cinc-1 levels in rat brain after meningitis induced by Streptococcus pneumoniae. J. Neuroimmunol., 2010-03-03;221(1):42-5. 2010-03-03 [PMID: 20202693] (Rat)
• Rhee CK, Kang CM, You MB, Yoon HK, Kim YK, Kim KH, Moon HS, Park SH, Song JS Effect of fudosteine on mucin production. Eur. Respir. J., 2008-06-25;32(5):1195-202. 2008-06-25 [PMID: 18579549] (Rat)
• Birrell MA, Catley MC, Hardaker E, Wong S, Willson TM, McCluskie K, Leonard T, Farrow SN, Collins JL, Haj-Yahia S, Belvisi MG Novel role for the liver X nuclear receptor in the suppression of lung inflammatory responses. J. Biol. Chem., 2007-08-31;282(44):31882-90. 2007-08-31 [PMID: 17766241] (Rat)
• Yang SR, Wright J, Bauter M, Seweryniak K, Kode A, Rahman I Sirtuin regulates cigarette smoke-induced proinflammatory mediator release via RelA/p65 NF-kappaB in macrophages in vitro and in rat lungs in vivo: implications for chronic inflammation and aging. Am. J. Physiol. Lung Cell Mol. Physiol., 2006-10-13;292(2):L567-76. 2006-10-13 [PMID: 17041012] (Rat)
• Birrell MA, McCluskie K, Hardaker E, Knowles R, Belvisi MG Utility of exhaled nitric oxide as a noninvasive biomarker of lung inflammation in a disease model. Eur. Respir. J., 2006-09-27;28(6):1236-44. 2006-09-27 [PMID: 17005583] (Rat)
• Frank JA, Wray CM, McAuley DF, Schwendener R, Matthay MA Alveolar macrophages contribute to alveolar barrier dysfunction in ventilator-induced lung injury. Am. J. Physiol. Lung Cell Mol. Physiol., 2006-07-28;291(6):L1191-8. 2006-07-28 [PMID: 16877636] (Rat)
• Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis. Arthritis Rheum., 2006-08-01;54(8):2402-14. 2006-08-01 [PMID: 16869003] (Rat)
• Watts JA, Zagorski J, Gellar MA, Stevinson BG, Kline JA Cardiac inflammation contributes to right ventricular dysfunction following experimental pulmonary embolism in rats. J. Mol. Cell. Cardiol., 2006-06-30;41(2):296-307. 2006-06-30 [PMID: 16814320] (Rat)
• Haddad el-B, McCluskie K, Birrell MA, Dabrowski D, Pecoraro M, Underwood S, Chen B, De Sanctis GT, Webber SE, Foster ML, Belvisi MG Differential effects of ebselen on neutrophil recruitment, chemokine, and inflammatory mediator expression in a rat model of lipopolysaccharide-induced pulmonary inflammation. J. Immunol., 2002-07-15;169(2):974-82. 2002-07-15 [PMID: 12097404] (Rat)

FAQs for CXCL1/GRO alpha/KC/CINC-1 (DY515). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Cxcl1