Rap2B Antibody (4F12-3C6) - Azide and BSA Free

Western Blot: Rap2B Antibody (4F12-3C6) [H00005912-M01] - RAP2B monoclonal antibody (M01), clone 4F12-3C6 Analysis of RAP2B expression in A-431.

Immunocytochemistry/Immunofluorescence: Rap2B Antibody (4F12-3C6) [H00005912-M01] - Analysis of monoclonal antibody to RAP2B on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Rap2B Antibody (4F12-3C6) [H00005912-M01] - Analysis of monoclonal antibody to RAP2B on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 1 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4F12-3C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Rap2B Antibody (4F12-3C6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RAP2B (AAH12362, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MREYKVVVLGSGGVGKSALTVQFVTGSFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYERVPMILVGNKVDLEGEREVSYGEGKALAEEWSCPFMETSAKNKASVDELFAEIVRQMNYAAQPNGDEGCCSACVIL
• Localization: Cell membrane. Lipid-anchor; Cytoplasmic side.
• Specificity: RAP2B - RAP2B, member of RAS oncogene family
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAP2B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recominant protein and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rap2B Antibody (4F12-3C6) - Azide and BSA Free

• MGC20484
• Rap2B
• RAP2B, member of RAS oncogene family
• Ras family small GTP binding protein RAP2B
• ras-related protein Rap-2b
• small GTP binding protein

Background

This intronless gene belongs to a family of RAS-related genes. The proteins encoded by these genes share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAP2B
• Entrez:
  • 5912(Human)
• OMIM:
  • 179541(Human)
• Uniprot:
  • AAH12362(Human)