RAP1GAP Antibody

Western Blot: RAP1GAP Antibody [NBP3-13211] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RAP1GAP antibody (NBP3-13211) diluted at 1:2000.

Rap1GAP antibody detects Rap1GAP protein at Golgi apparatus by immunofluorescent analysis.Sample: SK-N-AS cells were fixed in ice-cold MeOH for 5 min.Green: Rap1GAP protein stained by Rap1GAP antibody (NBP3-13211) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RAP1GAP Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse RAP1GAP. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAP1GAP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for RAP1GAP Antibody

• GTPase activating protein 1
• RAP1 GTPase activating protein

Background

Rap1GAP, an 85/95kDa protein, is a member of a family of GAP's specific for Rap1 (1). Though to be an important tumor suppressor, Rap1GAP has no sequence homology with other GAPs such as RasGAP, RanGAP and RhoGAP. Purified as a cytosolic and membrane-bound protein, both isoforms consist of two monomers each contain a dimerization and a catalytic domain (2-4). Mutations in the GAP domain of the tumor suppressor tuberin, can cause benign tumor phenotype tuberous sclerosis (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAP1GAP