RAP1GAP Antibody

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Western Blot: RAP1GAP Antibody [NBP1-33710] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE, antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.51 mg/ml

Order Details

RAP1GAP Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Rap1GAP. The exact sequence is proprietary.
Localization
Golgi apparatus membrane, Peripheral membrane protein
Predicted Species
Mouse (97%), Rat (98%), Bovine (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAP1GAP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.51 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RAP1GAP Antibody

  • GTPase activating protein 1
  • RAP1 GTPase activating protein

Background

Rap1GAP, an 85/95kDa protein, is a member of a family of GAP's specific for Rap1 (1). Though to be an important tumor suppressor, Rap1GAP has no sequence homology with other GAPs such as RasGAP, RanGAP and RhoGAP. Purified as a cytosolic and membrane-bound protein, both isoforms consist of two monomers each contain a dimerization and a catalytic domain (2-4). Mutations in the GAP domain of the tumor suppressor tuberin, can cause benign tumor phenotype tuberous sclerosis (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAP1GAP