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![Western Blot: Rap1A/B Antibody (6C7G0) [NBP3-16868] - Western blot analysis of extracts of various cell lines, using Rap1A/B Rabbit mAb (NBP3-16868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Rap1A-B-Antibody-6C7G0-Western-Blot-NBP3-16868-img0004.jpg "Western Blot: Rap1A/B Antibody (6C7G0) [NBP3-16868] - Western blot analysis of extracts of various cell lines, using Rap1A/B Rabbit mAb (NBP3-16868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Rap1A/B Antibody (6C7G0) [NBP3-16868] - Immunofluorescence analysis of U-2 OS cells using Rap1A/B Rabbit mAb (NBP3-16868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Rap1A-B-Antibody-6C7G0-Immunocytochemistry-Immunofluorescence-NBP3-16868-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Rap1A/B Antibody (6C7G0) [NBP3-16868] - Immunofluorescence analysis of C6 cells using Rap1A/B Rabbit mAb (NBP3-16868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Rap1A-B-Antibody-6C7G0-Immunocytochemistry-Immunofluorescence-NBP3-16868-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Rap1A/B Antibody (6C7G0) [NBP3-16868] - Immunofluorescence analysis of NIH-3T3 cells using Rap1A/B Rabbit mAb (NBP3-16868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Rap1A-B-Antibody-6C7G0-Immunocytochemistry-Immunofluorescence-NBP3-16868-img0002.jpg)
![Western Blot: Rap1A/B Antibody (SR1333) [NBP3-22136] - Western blot analysis using NBP3-22136 in Hela cell lysate.](http://images.novusbio.com/fullsize/nbp3-22136_rabbit-rap1a-b-mab-sr1333-6720239533714.jpg "Western Blot: Rap1A/B Antibody (SR1333) [NBP3-22136] - Western blot analysis using NBP3-22136 in Hela cell lysate.")
![Western Blot: Rap1A/B Antibody (JE35-61) [NBP3-32893] - Western blot analysis of Rap1A/B on different lysates with Rabbit anti-Rap1A/B antibody (NBP3-32893) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32893) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32893_rabbit-rap1a-b-mab-je35-61-288202412461669.jpg "Western Blot: Rap1A/B Antibody (JE35-61) [NBP3-32893] - Western blot analysis of Rap1A/B on different lysates with Rabbit anti-Rap1A/B antibody (NBP3-32893) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32893) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: Rap1A/B Antibody (JE35-61) [NBP3-32893] - Flow cytometric analysis of HEK-293 cells labeling Rap1A/B. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32893, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32893_rabbit-rap1a-b-mab-je35-61-29202416372167.jpg)
![Western Blot: Rap1A/B Antibody (SR2481) [NBP3-27757] - Western blot analysis of Rap1A/B in K562, rat Brain, C6, 3T3, Hela lysates](http://images.novusbio.com/fullsize/nbp3-27757_rabbit-rap1a-b-mab-sr2481-75202410133459.jpg "Western Blot: Rap1A/B Antibody (SR2481) [NBP3-27757] - Western blot analysis of Rap1A/B in K562, rat Brain, C6, 3T3, Hela lysates")