![Western Blot: RanBP2 Antibody (2E1) [NB100-74480] - Analysis of 25ug of cytoplasmic and nuclear HeLa cell lysates prepared using NE-PER Nuclear and Cytoplasmic Extraction Reagents per well, 16ug of HeLa whole cell lysate prepared using M-PER Mammalian Protein Extraction Reagent, and 10ul of PageRuler Plus Prestained Protein Ladder.](http://images.novusbio.com/fullsize/RanBP2-Antibody-2E1-Western-Blot-NB100-74480-img0003.jpg "Western Blot: RanBP2 Antibody (2E1) [NB100-74480] - Analysis of 25ug of cytoplasmic and nuclear HeLa cell lysates prepared using NE-PER Nuclear and Cytoplasmic Extraction Reagents per well, 16ug of HeLa whole cell lysate prepared using M-PER Mammalian Protein Extraction Reagent, and 10ul of PageRuler Plus Prestained Protein Ladder.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 2E1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant protein corresponding to 150 N-terminal residues of human Nup358. |
| Localization | Nuclear |
| Specificity | Nup358 (2E1) |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RANBP2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Publications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 1 mg/ml BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
| Publication using NB100-74480 | Applications | Species |
|---|---|---|
| Rossi A, Coum A, Madelenat M, et al. Neural stem cells alter nucleocytoplasmic partitioning and accumulate nuclear polyadenylated transcripts during quiescence bioRxiv 2021-01-07 (WB) | WB |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: RanBP2 Antibody (2E1) [NB100-74480] - Analysis of Nup358 (green) in HeLa cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 1% Blocker BSA in PBS, each for 15 minutes at room temperature. Cells were stained with a Nup358 monoclonal antibody at a concentration of 10ug/ml in 1% Blocker BSA in PBS, followed by a DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.](http://images.novusbio.com/fullsize/RanBP2-Antibody-2E1-Immunocytochemistry-Immunofluorescence-NB100-74480-img0002.jpg "Immunocytochemistry/Immunofluorescence: RanBP2 Antibody (2E1) [NB100-74480] - Analysis of Nup358 (green) in HeLa cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 1% Blocker BSA in PBS, each for 15 minutes at room temperature. Cells were stained with a Nup358 monoclonal antibody at a concentration of 10ug/ml in 1% Blocker BSA in PBS, followed by a DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.")
![Immunohistochemistry-Paraffin: RanBP2 Antibody (2E1) [NB100-74480] - Normal biopsies of deparaffinized human stomach tissue.](http://images.novusbio.com/fullsize/RanBP2-Antibody-2E1-Immunohistochemistry-Paraffin-NB100-74480-img0001.jpg "Immunohistochemistry-Paraffin: RanBP2 Antibody (2E1) [NB100-74480] - Normal biopsies of deparaffinized human stomach tissue.")
