RanBP2 Antibody (2E1)

Images

 
Western Blot: RanBP2 Antibody (2E1) [NB100-74480] - Analysis of 25ug of cytoplasmic and nuclear HeLa cell lysates prepared using NE-PER Nuclear and Cytoplasmic Extraction Reagents per well, 16ug of HeLa whole cell ...read more
Immunocytochemistry/ Immunofluorescence: RanBP2 Antibody (2E1) [NB100-74480] - Analysis of Nup358 (green) in HeLa cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and ...read more
Immunohistochemistry-Paraffin: RanBP2 Antibody (2E1) [NB100-74480] - Normal biopsies of deparaffinized human stomach tissue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
2E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RanBP2 Antibody (2E1) Summary

Immunogen
Recombinant protein corresponding to 150 N-terminal residues of human Nup358.
Localization
Nuclear
Specificity
Nup358 (2E1)
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RANBP2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:1000
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for RanBP2 Antibody (2E1)

  • ANE1
  • E3 SUMO-protein ligase RanBP2
  • EC 5.2.1.8
  • Nuclear pore complex protein Nup358
  • nucleoporin 358
  • Nucleoporin Nup358
  • NUP358TRP2
  • p270
  • RAN binding protein 2
  • Ran-binding protein 2
  • RanBP2
  • transformation-related protein 2,358 kDa nucleoporin
  • TRP1

Background

Nup358 is an essential protein needed for proper kinetochore function. Chromatin congregation and segregation are distributed in the absence of Nup358. The assembly of other kinetochore components is also strongly inhibited. Therefore, Nup358 plays an essential role in the association of kinetochore function and maturation and nuclear envelope breakdown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RANBP2
Entrez
Uniprot