Rad21 Antibody (BLR052F)

Western Blot: Rad21 Antibody [NBP3-14677] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 cellsprepared using NETN lysis buffer. Antibody: Rabbit antiRad21 recombinant monoclonal antibody used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG.Chemiluminescence with an exposure time of 3 seconds.

Western Blot: Rad21 Antibody [NBP3-14677] - Whole cell lysate (50 ug) from HeLa, Jurkat, HEK293T, RKO,GaMG, MCF-7, A-549, and U2OS cells prepared usingNETN lysis buffer. Antibody: Rabbit anti-Rad21recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goatanti-rabbit IgG. Chemiluminescence with anexposure time of 3 seconds. Lower panel: Rabbit antiMED1 recombinant monoclonal antibody.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: BLR052F
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Rad21 Antibody (BLR052F) Summary

• Description: Novus Biologicals Rabbit Rad21 Antibody (BLR052F) (NBP3-14677) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal
• Immunogen: between 581 and 631 (C-term)
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RAD21
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
• Preservative: 0.09% Sodium Azide
• Purity: >95%

Alternate Names for Rad21 Antibody (BLR052F)

• double-strand-break repair protein rad21 homolog
• hHR21FLJ25655
• HR21
• KIAA0078RAD21 (S. pombe) homolog
• MCD1
• Nuclear matrix protein 1
• NXP-1
• NXP1HRAD21
• protein involved in DNA double-strand break repair
• RAD21 homolog (S. pombe)
• SCC1 homolog
• SCC1FLJ40596

Background

The cohesin complex plays an important role in tethering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys the cohesin complex by means of cleavaging its Scc1 subunit (Mcd1/Scc1/hRad21), allowing the chromatids to separate and divide with the cell. During apoptosis the C-terminal portion of hRad21 is cleaved and translocated to the cytoplasm. This cleavage and translocation may act as a nuclear signal that promotes and accelerates subsequent events of apoptosis (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAD21