Rab7a Antibody [Alexa Fluor® 700]

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

Rab7a Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human Rab7a
  Thr2-Ser204
  Accession # P51149
• Specificity: Detects human and mouse Rab7a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Rab9a and rhRab9b is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rab7a Antibody [Alexa Fluor® 700]

• CMT2B
• FLJ20819
• member RAS oncogene family
• PRO2706
• PSN
• RAB7
• Rab7a
• RAB7A, member RAS oncogene family
• Ras-associated protein RAB7
• ras-related protein Rab-7a

Background

Rab7a (Ras-related protein Rab 7A; also RAB7) is a 22-24 kDa member of the Rab family, Small GTPase superfamily of proteins. It is widely expressed, and found in cells diverse as skeletal muscle, neurons, osteoclasts and macrophages. Rab7a plays a key role in the formation of phagolysosomes. In particular, Rab7a-GTP becomes associated with early phagosomes. The GTP form of Rab7a first recruits RILP, a molecule that interacts with ORPL1 and promotes the formation of a dynactin:dynein complex. This complex now initiates the movement of the phagosome along microtubules, where it fuses with lysosomes and endosomes at select points, creating phagolysosomes. Human Rab7a is 207 amino acids (aa) in length. It contains multiple Rab family and subfamily motifs, and concludes with a C‑terminal CXC prenylation sequence (aa 205-207). There is one utilized phosphorylation site at Tyr183. Full-length human and mouse Rab7a have identical aa sequences. Notably, however, point mutations with single aa changes are noted in both species. By contrast, human Rab7a and Rab7b share less than 50% aa sequence identity, each representing the product of distinct genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.