Rab5a Antibody (5D1)

Western Blot: Rab5a Antibody (5D1) [H00005868-M09A] - Analysis of RAB5A expression in K-562 (Cat # L009V1).

Product Discontinued

Rab5a Antibody (5D1) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RAB5A (AAH01267, 116 a.a. ~ 215 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN
• Marker: Early Endosome Marker
• Specificity: RAB5A - RAB5A, member RAS oncogene family (5D1)
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAB5A
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rab5a Antibody (5D1)

• RAB5A, member RAS oncogene family
• RAB5RAS-associated protein RAB5A
• ras-related protein Rab-5A

Background

Rab5 is a 24 kDa GTP-binding protein that regulates the fusion of plasma membrane -derived clathrin-coated vesicles with early endosomes , and homotypic fusion among early endosomes1. Localized to the cytoplasmic side of the plasma membrane, clathrin -coated vesicles and early endosomes, Rab5 appears to regulate vesicle fusion through a cycle of GDP/GTP e xchange and GTP hydrolysis. The different guanine nuc leotide binding states of Rab5 may affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. The GTP-bound, or active, form of Rab5 associates with membrane s and regulates vesicle docking and fusion. Studies using a Rab5 mutant that hydrolyzed xanthosine 5 -triphosphate (XTP) indicated that nucleotide hydrolysis occurs even in the absence of membrane fusion. GTP hydrolysis by Rab5 may determine the frequency of membrane docking and fusion events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rab5a Antibody (H00005868-M09A)(1)

Publication using H00005868-M09A

• Chamberlain MD, Oberg JC, Furber LA et al. Deregulation of Rab5 and Rab4 proteins in p85R274A-expressing cells alters PDGFR trafficking. Cell Signal 22(10):1562-75. 2010-10-01 [PMID: 20570729]

Bioinformatics

• Gene Symbol: RAB5A
• Entrez:
  • 5868(Human)
• Uniprot:
  • AAH01267(Human)