Rab17 Antibody (2A10) - Azide and BSA Free

Immunohistochemistry-Paraffin: Rab17 Antibody (2A10) [H00064284-M01] - Analysis of monoclonal antibody to RAB17 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.

Sandwich ELISA: Rab17 Antibody (2A10) [H00064284-M01] - Detection limit for recombinant GST tagged RAB17 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 2A10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Rab17 Antibody (2A10) - Azide and BSA Free Summary

• Immunogen: RAB17 (AAH50426, 1 a.a. ~ 212 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAQAHRTPQPRAAPSQPRVFKLVLLGSGSVGKSSLALRYVKNDFKSILPTVGCAFFTKVVDVGATSLKLEIWDTAGQEKYHSVCHLYFRGANAALLVYDITRKDSFLKAQQWLKDLEEELHPGEVLVMLVGNKTDLSQEREVTFQEGKEFADSQKLLFMETSAKLNHQVSEVFNTVAQELLQRSDEEGQALRGDAAVALNKGPARQAKCCAH
• Specificity: RAB17 - RAB17, member RAS oncogene family
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAB17
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:502
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. Has also been used for immunohistochemistry (paraffin).

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rab17 Antibody (2A10) - Azide and BSA Free

• FLJ12538
• RAB17, member RAS oncogene family
• ras-related protein Rab-17

Background

The Rab subfamily of small GTPases plays an important role in the regulation of membrane trafficking. RAB17 is anepithelial cell-specific GTPase (Lutcke et al., 1993 (PubMed 8486736)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rab17 Antibody (H00064284-M01)(1)

Publication using H00064284-M01

• Sergi M, Matthew N, Madeleine M et al. Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion. Nat Commun. 2021-11-24 [PMID: 34819513]

Bioinformatics

• Gene Symbol: RAB17
• Entrez:
  • 64284(Human)
• Uniprot:
  • AAH50426(Human)