Rab11A Antibody (918009) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HT1080 human fibrosarcoma cell line, C2C12 mouse myoblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Rab11A Monoclonal Antibody (Catalog # MAB8978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Rab11A at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 918009
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Rab11A Antibody (918009) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Rab-11A
  Met1-Glu162
  Accession # P62491
• Specificity: Detects human Rab-11A in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAB11A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rab11A Antibody (918009) [Unconjugated]

• MGC1490
• RAB 11A, member oncogene family
• RAB11
• Rab-11
• Rab11A
• RAB11A, member RAS oncogene family
• YL8
• YL8ras-related protein Rab-11A

Background

Ras-related protein Rab11A is a 25 kDa member of the Rab family of small GTPases.  With more than 40 family members, Rab proteins are regulators of vesicular transport, and their number reflects the complexity of vesicle trafficking.  Rab family members cycle between an inactive GDP-bound form and an active GTP-bound form that recruits to membranes various downstream effectors directly responsible for vesicle formation, migration and fusion.  Rab11A is ubiquitously expressed, with Northern blot analysis detecting highest levels in heart, and lowest levels in liver.  Human Rab11A is 216 amino acids (aa) in length, with residues 214-216 removed in its mature form.  Over aa 1-162, human Rab11A shares 100% identity with mouse and rat Rab11A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Rab11A Antibody (MAB8978) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/20/2017

Summary

• Application: Western Blot
• Sample Tested: MDA-MB-231 Cell Lysate
• Species: Human

Comments

• Comments: a few non-specific bands above 50kDa.

Bioinformatics

• Gene Symbol: RAB11A
• Uniprot:
  • P62491()