Rab11A Antibody (1F7-2A8) - Azide and BSA Free

Western Blot: Rab11A Antibody (1F7-2A8) [H00008766-M01] - RAB11A monoclonal antibody (M01), clone 1F7-2A8. Analysis of RAB11A expression in HepG2.

Western Blot: Rab11A Antibody (1F7-2A8) [H00008766-M01] - RAB11A monoclonal antibody (M01), clone 1F7-2A8. Analysis of RAB11A expression in PC-12.

Western Blot: Rab11A Antibody (1F7-2A8) [H00008766-M01] - RAB11A monoclonal antibody (M01), clone 1F7-2A8 Analysis of RAB11A expression in K-562.

Sandwich ELISA: Rab11A Antibody (1F7-2A8) [H00008766-M01] - Detection limit for recombinant GST tagged RAB11A is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA
• Clone: 1F7-2A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Rab11A Antibody (1F7-2A8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RAB11A (AAH13348.1, 1 a.a. ~ 216 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI
• Localization: Cytoplasmic
• Marker: Recycling Endosome Marker
• Specificity: RAB11A - RAB11A, member RAS oncogene family
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAB11A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rab11A Antibody (1F7-2A8) - Azide and BSA Free

• MGC1490
• RAB 11A, member oncogene family
• RAB11
• Rab-11
• Rab11A
• RAB11A, member RAS oncogene family
• YL8
• YL8ras-related protein Rab-11A

Background

Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There are over 40 Rab proteins in mammals. Rab11a and Rab11b are known markers for protein trafficking, sorting, and recycling in the endosomal pathway. The Rab11 proteins are enriched in recycling endosomes and the trans-Golgi network, where they regulate membrane recycling back to the plasma membrane. Rab11a is ubiquitously expressed, while Rab11b is expressed mainly in the heart and brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAB11A
• Entrez:
  • 8766(Human)
• OMIM:
  • 605570(Human)
• Uniprot:
  • AAH13348(Human)