RAB10 Antibody (4E2)

Western Blot: RAB10 Antibody (4E2) [NBP1-51634] - Analysis using Rab10 mouse mAb against Hela (1) and NIH/3T3 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: RAB10 Antibody (4E2) [NBP1-51634] - Immunofluorescence analysis of LOVO cells using Rab10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 4E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

RAB10 Antibody (4E2) Summary

• Description: Novus Biologicals Mouse RAB10 Antibody (4E2) (NBP1-51634) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human RAB10 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAB10
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 22.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for RAB10 Antibody (4E2)

• GTP-binding protein RAB10
• RAB10, member RAS oncogene family
• ras-related GTP-binding protein
• ras-related protein Rab-10

Background

RAB10, member RAS oncogene family. The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAB10