R-Spondin 1 Antibody (422407) [Alexa Fluor® 750]

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 422407
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

R-Spondin 1 Antibody (422407) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human R-Spondin 1
  Met1-Gly182
  Accession # NP_775911
• Specificity: Detects human and mouse R-Spondin 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) R-Spondin 2 or rhR-Spondin 3.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for R-Spondin 1 Antibody (422407) [Alexa Fluor® 750]

• Cristin 3
• CRISTIN3
• FLJ40906Roof plate-specific spondin-1
• HRspo1
• roof plate-specific spondin
• RSPO
• RSPO1
• RSpondin 1
• R-Spondin 1
• R-spondin homolog (Xenopus laevis)
• RSPONDIN
• R-spondin1
• R-spondin-1

Background

RSPO-1 (roof plate-specific spondin; also R-spondin1 and cristin3) is a 39 kDa, heparin-binding member of the Cristin/R-spondin family of proteins. These molecules apparently are either retained intracellularly or secreted, where they activate the frizzled-LRP receptor pathway. Mature human RSPO-1 is 27 kDa and 243 amino acids (aa) in length. It contains a cysteine-rich furin repeat domain followed by a type I TSP domain. This form is apparently retained intracellularly. A presumably glycosylated, 30 kDa, 193 aa C-terminally truncated form is secreted and interacts with heparin. The secreted form of human RSPO-1 is 93% aa identical to secreted mouse RSPO-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.