Pyruvate Dehydrogenase E2 Antibody

Western Blot: Pyruvate Dehydrogenase E2 Antibody [H00001737-B01P] - Analysis of DLAT expression in rat brain.

Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase E2 Antibody [H00001737-B01P] - Analysis of purified antibody to DLAT on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: Pyruvate Dehydrogenase E2 Antibody [H00001737-B01P] - Analysis of DLAT expression in HepG2.

Western Blot: Pyruvate Dehydrogenase E2 Antibody [H00001737-B01P] - Analysis of DLAT expression in transfected 293T cell line by DLAT polyclonal antibody. Lane 1: DLAT transfected lysate(71.17 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

Pyruvate Dehydrogenase E2 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: DLAT (NP_001922.2, 1 a.a. - 647 a.a.) full-length human protein. MWRVCARRAQNVAPWAGLEARWTALQEVPGTPRVTSRSGPAPARRNSVTTGYGGVRALCGWTPSSGATPRNRLLLQLLGSPGRRYYSLPPHQKVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPTPPPVAAVPPTPQPLAPTPSAPCPATPAGPKGRVFVSPLAKKLAVEKGIDLTQVKGTGPDGRITKKDIDSFVPSKVAPAPAAVVPPTGPGMAPVPTGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLSIDVNMGEVLLVRKELNKILEGRSKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGVETIANDVVSLATKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLVPADNEKGFDVASMMSVTLSCDHRVVDGAVGAQWLAEFRKYLEKPITMLL
• Specificity: Reacts with dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: DLAT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This antibody is reactive against tissue and transfected lysate in WB, IF and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Pyruvate Dehydrogenase E2 Antibody

• 70 kDa mitochondrial autoantigen of primary biliary cirrhosis
• dihydrolipoamide S-acetyltransferase
• DLTA
• E2 component of pyruvate dehydrogenase complex
• EC 2.3.1
• M2 antigen complex 70 kDa subunit
• PBC
• PDC-E2EC 2.3.1.12
• PDCE2mitochondrial

Background

The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLAT
• Entrez:
  • 1737(Human)
• Uniprot:
  • NP_001922.2(Human)