Pyruvate Dehydrogenase E1 beta subunit Antibody (JM66-33)

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (JM66-33) [NBP2-75639] - Analysis of Pyruvate Dehydrogenase E1 beta subunit on mouse kidney tissue (1) and PC-12 (2) cell lysate using anti-Pyruvate Dehydrogenase E1 beta subunit antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody (JM66-33) [NBP2-75639] - Analysis of paraffin-embedded rat heart tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin.

Flow Cytometry: Pyruvate Dehydrogenase E1 beta subunit Antibody (JM66-33) [NBP2-75639] - Analysis of Hela cells with Pyruvate Dehydrogenase E1 beta subunit antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (JM66-33) [NBP2-75639] - Analysis of Pyruvate Dehydrogenase E1 beta subunit on HepG2 (1) and Hela (2) cell lysate using anti-Pyruvate Dehydrogenase E1 beta subunit antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody (JM66-33) [NBP2-75639] - Analysis of paraffin-embedded human stomach cancer tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody (JM66-33) [NBP2-75639] - Analysis of paraffin-embedded human kidney tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody (JM66-33) [NBP2-75639] - Analysis of paraffin-embedded mouse brain tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody (JM66-33) [NBP2-75639] - Analysis of paraffin-embedded mouse hippocampus tissue using anti-Pyruvate Dehydrogenase E1 beta subunit antibody. Counter stained with hematoxylin.

Product Discontinued

Pyruvate Dehydrogenase E1 beta subunit Antibody (JM66-33) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Pyruvate Dehydrogenase E1 beta subunit aa 310-359 / 359. (SwissProt: P11177 Human; SwissProt: Q9D051 Mouse; SwissProt: P49432 Rat)
• Localization: Mitochondrion matrix.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PDHB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Pyruvate Dehydrogenase E1 beta subunit Antibody (JM66-33)

• DKFZp564K0164
• mitochondrial
• pyruvate dehydrogenase (lipoamide) beta
• pyruvate dehydrogenase, E1 beta polypeptide

Background

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDHB