Rat Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric)

ELISA: Rat Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric) [NBP2-81221] - Samples were spiked with high concentrations of Rat Pyruvate Dehydrogenase E1-alpha subunit and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric) [NBP2-81221] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL

Rat Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat Pyruvate Dehydrogenase E1-alpha subunit in samples. No significant cross-reactivity or interference between Rat Pyruvate Dehydrogenase E1-alpha subunit and analogues was observed.
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.95%
• Intra-Assay: CV% < 5.59%
• Spike Recovery: 89-105%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PDHA1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric)

• EC 1.2.4.1
• PDHA
• PDHE1-A type I
• PHE1APDHCE1A
• pyruvate dehydrogenase (lipoamide) alpha 1
• pyruvate dehydrogenase complex, E1-alpha polypeptide 1
• pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Background

Pyruvate Dehydrogenase E1-alpha subunit (PDHE1 alpha) is a member of the pyruvate dehydrogenase complex (PDC) and is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1 or PDHE1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex. Enhanced expression of pyruvate dehydrogenase kinase-1 (PDK-1) results in phosphorylation of PDHE1 alpha at Serine 293, which in turn produces nearly complete inhibition of the pyruvate dehydrogenase complex (PDC). A recent study shows that inhibition of PDC activity in cancer cells restores normoxic stablization of HIF-1 alpha by glycolytic metabolites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (NBP2-81221). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PDHA1