Rat Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric)

ELISA: Rat Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric) [NBP2-81221] - Samples were spiked with high concentrations of Rat Pyruvate Dehydrogenase E1-alpha subunit and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric) [NBP2-81221] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL

Rat Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat Pyruvate Dehydrogenase E1-alpha subunit in samples. No significant cross-reactivity or interference between Rat Pyruvate Dehydrogenase E1-alpha subunit and analogues was observed.
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.95%
• Intra-Assay: CV% < 5.59%
• Spike Recovery: 89-105%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PDHA1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Pyruvate Dehydrogenase E1-alpha subunit ELISA Kit (Colorimetric)

• EC 1.2.4.1
• PDHA
• PDHE1-A type I
• PHE1APDHCE1A
• pyruvate dehydrogenase (lipoamide) alpha 1
• pyruvate dehydrogenase complex, E1-alpha polypeptide 1
• pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Background

Pyruvate Dehydrogenase E1-alpha subunit (PDHE1 alpha) is a member of the pyruvate dehydrogenase complex (PDC) and is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1 or PDHE1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex. Enhanced expression of pyruvate dehydrogenase kinase-1 (PDK-1) results in phosphorylation of PDHE1 alpha at Serine 293, which in turn produces nearly complete inhibition of the pyruvate dehydrogenase complex (PDC). A recent study shows that inhibition of PDC activity in cancer cells restores normoxic stablization of HIF-1 alpha by glycolytic metabolites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PDHA1