Pyruvate Dehydrogenase E1-alpha subunit Antibody

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [H00005160-B01P] - Analysis of PDHA1 expression in rat brain.

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [H00005160-B01P] - Analysis of purified antibody to PDHA1 on formalin-fixed paraffin-embedded human stomach. (antibody concentration 1.5 ug/ml)

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [H00005160-B01P] - PDHA1 MaxPab polyclonal antibody. Western Blot analysis of PDHA1 expression in human liver.

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [H00005160-B01P] - Analysis of PDHA1 expression in HeLa.

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [H00005160-B01P] - Analysis of PDHA1 expression in HepG2.

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [H00005160-B01P] - Analysis of PDHA1 expression in NIH/3T3.

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [H00005160-B01P] - Analysis of PDHA1 expression in human kidney.

Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [H00005160-B01P] - Analysis of PDHA1 expression in transfected 293T cell line by PDHA1 polyclonal antibody. Lane 1: PDHA1 transfected lysate(43.01 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

Pyruvate Dehydrogenase E1-alpha subunit Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: PDHA1 (AAH02406, 1 a.a. - 390 a.a.) full-length human protein. MRKMLAAVSRVLSGASQKPASRVLVASRNFANDATFEIKKCDLHRLEEGPPVTTVLTREDGLKYYRMMQTVRRMELKADQLYKQKIIRGFCHLCDGQEACCVGLEAGINPTDHLITAYRAHGFTFTRGLSVREILAELTGRKGGCAKGKGGSMHMYAKNFYGGNGIVGAQVPLGAGIALACKYNGKDEVCLTLYGDGAANQGQIFEAYNMAALWKLPCIFICENNRYGMGTSVERAAASTDYYKRGDFIPGLRVDGMDILCVREATRFAAAYCRSGKGPILMELQTYRYHGHSMSDPGVSYRTREEIQEVRSKSDPIMLLKDRMVNSNLASVEELKEIDVEVRKEIEDAAQFATADPEPPLEELGYHIYSSDPPFEVRGANQWIKFKSVS
• Specificity: PDHA1 - pyruvate dehydrogenase (lipoamide) alpha 1,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: PDHA1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Antibody reactivity against transfected lysate and tissue lysate for WB. It has been used for IHC-P and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Pyruvate Dehydrogenase E1-alpha subunit Antibody

• EC 1.2.4.1
• PDHA
• PDHE1-A type I
• PHE1APDHCE1A
• pyruvate dehydrogenase (lipoamide) alpha 1
• pyruvate dehydrogenase complex, E1-alpha polypeptide 1
• pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial

Background

The pyruvate dehydrogenase complex is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1); dihydrolipoyl transacetylase (DLAT; MIM 608770) (E2; EC 2.3.1.12); and dihydrolipoyl dehydrogenase (DLD; MIM 238331) (E3; EC 1.8.1.4). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex (Brown et al., 1994 [PubMed 7853374]).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDHA1
• Entrez:
  • 5160(Human)
• Uniprot:
  • AAH02406(Human)