Home » PUS1 » PUS1 Antibodies » PUS1 Antibody

PUS1 Antibody

 Submit a Review

Images

 
(
Enlarge)
Western Blot: PUS1 Antibody [NB100-86983] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-86983 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.
Independent Antibodies: Western Blot: PUS1 Antibody [NB100-86983] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-86983 used for WB at 0.04 mcg/ml (A) and 1 ...read more
Immunohistochemistry: PUS1 Antibody [NB100-86983] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PUS1 used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

        

Order Details

100% Guarantee on every product

PUS1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 377 and 427 of human pseudouridylate synthase 1 using the numbering given in entry NP_079491.2 (GeneID 80324).
Specificity
This antibody detects both isoforms of PUS1: Isoform 1 (47.5 kDa) and Isoform 2 (44.4kDa).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PUS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections
Control
PUS1 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PUS1 Antibody

  • EC 5.4.99.12
  • MGC11268
  • mitochondrial tRNA pseudouridine synthase A
  • MLASA1
  • pseudouridylate synthase 1
  • tRNA pseudouridine synthase A, mitochondrial
  • tRNA pseudouridylate synthase I
  • tRNA uridine isomerase I
  • tRNA-uridine isomerase I

Background

PUS1 converts uridine into pseudouridine after the nucleotide has been incorporated into RNA. Pseudouridine may have a functional role in tRNAs and may assist in the peptidyl transfer reaction of rRNAs (Chen and Patton, 1999 [PubMed 10094309]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

S5a/Angiocidin
Antibody
NBP2-37888
Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Immunocytochemistry/Immunofluorescence: S5a/Angiocidin Antibody [NBP2-37888] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human cerebral cortex, liver, lymph node and testis using Anti-PSMD4 antibody NBP2-37888 (A) shows similar protein distribution across tissues to independent antibody NBP1-90821 (B).
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
p97/VCP Antibody
NB100-1558
Western Blot: p97/VCP Antibody [NB100-1558] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiVCP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Western Blot: p97/VCP Antibody [NB100-1558] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody NB100-1558 used for IP at 3 ug per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody NB100-1557. For blotting immunoprecipitated VCP, NB100-1558 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells and asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated and subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue and afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected and cropped by CiteAb from the following publication (http://biosignaling.biomedcentral.com/articles/10.1186/1478-811X-10-13) licensed under a CC-BY licence.
(10 Publications)
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-Fr, IP, WB
FECH Antibody
NBP2-33413
Immunohistochemistry-Paraffin: FECH Antibody [NBP2-33413] - Staining of human liver shows weak granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: FECH Antibody [NBP2-33413] - Staining of human prostate shows moderate granular cytoplasmicd positivity in glandular cells.
Immunohistochemistry-Paraffin: FECH Antibody [NBP2-33413] - Staining of human lymph node shows no positivity in germinal center cells as expected.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
Proteasome 19S 10B
Antibody
NB100-1483
Western Blot: Proteasome 19S 10B Antibody [NB100-1483] - Staining of HeLa (A), U251 (B), KNRK (C) with antibody at 1 ug/mL and NIH3T3 (D) cell lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody [NB100-1483] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: Proteasome 19S 10B Antibody [NB100-1483] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
(6 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, PEP-ELISA, PLA, WB
GLXR5 Antibody
NBP1-89897
Western Blot: GLXR5 Antibody [NBP1-89897] - Analysis in control (vector only transfected HEK293T lysate) and LY414008 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: GLXR5 Antibody [NBP1-89897] - Staining of human skeletal muscle shows strong granular cyotplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: GLXR5 Antibody [NBP1-89897] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and leydig cells.
Species: Hu
Applications: IHC, IHC-P, WB
ABCB7 Antibody
NBP2-92864
Immunocytochemistry/Immunofluorescence: ABCB7 Antibody [NBP2-92864] - Analysis of HeLa cells using ABCB7 . Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ABCB7 Antibody [NBP2-92864] - Paraffin-embedded human gastric cancer using ABCB7 .
Immunohistochemistry-Paraffin: ABCB7 Antibody [NBP2-92864] - Paraffin-embedded mouse brain using ABCB7 .
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
PSMD8 Antibody
NBP1-83254
Immunocytochemistry/Immunofluorescence: PSMD8 Antibody [NBP1-83254] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254] - Staining of human cerebellum shows moderate nuclear positivity in Purkinje cells.
Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
OVCA1 Antibody
NBP1-56693
Western Blot: OVCA1 Antibody [NBP1-56693] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.
Immunocytochemistry/Immunofluorescence: OVCA1 Antibody [NBP1-56693] - Formalin Fixed Paraffin; Embedded Tissue: Human Adult Liver; Observed Staining: Cytoplasm (perinuclear) but not nuclear in hepatocytes, strong signal, low tissue distribution; Primary Antibody Concentration: 1:100
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Ubiquilin 2
Antibody (5F5)
H00029978-M03
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - UBQLN2 shRNA knockdown in SH-SY5Y cells. First lane is the ladder, then treatment with scrambled shRNA and third lane is UBQLN2 knockdown. 30 ug of the protein sample was loaded on the gel and transferred to the nitrocellulose membrane. Overnight incubation in primary antibodies at 4 degrees C in 5 % milk in TBST. Then incubated in secondary antibodies (Goat Anti-mouse DyLight 550) at RT for 2 h, and then imaged. Knockdown of the upper band with shRNA for UBQLN2 was about 82 %, and of the lower band only about 8 %, suggesting a high specificity of the used shRNA. This image was submitted via customer Review.
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Generation of transgenic mice that overexpress human ubiquilin-1. Immunoblots of equal amounts of total brain lysates from 12 month-old WT mouse, 12 month-old Ubqln-1 48 transgenic mouse, 12 month-old Ubqln-1 62 transgenic mouse and end stage 15 week-old R6/2 transgenic mouse. The top panel was probed with a monoclonal anti-ubiquilin antibody (Invitrogen antibody clone 3D5E2) and the lower panel with a different monoclonal anti-Ubiquilin 2 antibody (Novus antibody clone 5F5). Note two immunoreactive ubiquilin bands are seen at 70 kDa and at 90 kDa, which we presume is a modified form of ubiquilin. Both blots were also probed for actin to ensure equal loading. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0087513) licensed under a CC-BY licence.
Western Blot: Ubiquilin 2 Antibody (5F5) [H00029978-M03] - Confirmation of polyQ-associated proteins from PC-12 cells identified by TAPI. Western blot analysis of TAPI-purified polyQ aggregates from PC-12 cells confirms the presence of several disease-associated proteins only in the Htt-Q74 samples. All proteins migrated near their predicted molecular weights. For control, the TAPI procedure was conducted in parallel on the induced Htt-Q23 cell line (FUS, TDP-43, UBQLN2, HNRNPA1) or the un-induced Htt-Q74 cell line (CLINT1, HSPA8, RAD23B, SGTA). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0136362), licensed under a CC-BY licence.
(1 Review)
(26 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, KD, WB
Ubiquilin 1
Antibody
NB120-3341
Western Blot: Ubiquilin 1 Antibody [NB120-3341] - Analysis of ubiquilin on CHO cell extract.
(1 Review)
Species: ChHa, Ha, Hu, Mu
Applications: WB
GFM1 Antibody
NBP1-83997
Immunohistochemistry-Paraffin: GFM1 Antibody [NBP1-83997] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: GFM1 Antibody [NBP1-83997] - Staining of human placenta shows high expression.
Immunohistochemistry-Paraffin: GFM1 Antibody [NBP1-83997] - Staining in human placenta and pancreas tissues using anti-GFM1 antibody. Corresponding GFM1 RNA-seq data are presented for the same tissues.
(1 Publication)
Species: Hu
Applications: IHC-P, WB
TRUB1 Antibody
NBP1-80866
Immunocytochemistry/Immunofluorescence: TRUB1 Antibody [NBP1-80866] - Staining of human cell line U-2 OS shows localization to actin filaments. Antibody staining is shown in green.
Western Blot: TRUB1 Antibody [NBP1-80866] - Analysis in control (vector only transfected HEK293T lysate) and TRUB1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: TRUB1 Antibody [NBP1-80866] - Staining of human testis shows moderate cytoplasmic positivity in a subset of cells in seminiferous ducts.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
PSMD14 Antibody
NBP1-80956
Immunohistochemistry-Paraffin: PSMD14 Antibody [NBP1-80956] - Staining of human hippocampus shows strong nuclear positivity in neuronal cells.
Western Blot: PSMD14 Antibody [NBP1-80956] - Analysis in human cell line HepG2.
Western Blot: PSMD14 Antibody [NBP1-80956] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
(4 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
SH2D3A Antibody
NBP1-82525
Western Blot: SH2D3A Antibody [NBP1-82525] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-523
Immunohistochemistry-Paraffin: SH2D3A Antibody [NBP1-82525] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: SH2D3A Antibody [NBP1-82525] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, WB
Proteasome 19S S3
Antibody (OTI2A2) - A ...
NBP2-73636
Western Blot: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 19S S3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western Blot: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S S3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Prot
Flow Cytometry: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636] - Analysis of Hela cells, using anti-Proteasome 19S S3 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: CyTOF-ready, Flow, WB
SCGF/CLEC11a
Antibody (239029) [Un ...
MAB1904
SCGF/CLEC11a was detected in immersion fixed IFN gamma activated THP-1 human acute monocytic leukemia cell line using 10 µg/mL Mouse Anti-Human SCGF/CLEC11a Monoclonal Antibody (Catalog # MAB1904) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
SCGF/CLEC11a was detected in immersion fixed paraffin-embedded sections of human spleen using 25 µg/mL Mouse Anti-Human SCGF/CLEC11a Monoclonal Antibody (Catalog # MAB1904) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(1 Publication)
Species: Hu
Applications: ICC, IHC, WB
Recombinant Human
AASDH GST (N-Term) Pr ...
H00132949-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
Applications: ELISA, AP, PA, WB
SLC25A38 Antibody
NBP2-93818
Western Blot: SLC25A38 Antibody [NBP2-93818] - Analysis of extracts of 293T cells, using SLC25A38 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure tim
Immunocytochemistry/Immunofluorescence: SLC25A38 Antibody [NBP2-93818] - Analysis of NIH/3T3 cells using SLC25A38 at dilution of 1:100. Blue: DAPI for nuclear staining.
Species: Hu, Mu
Applications: ICC/IF, WB

Publications for PUS1 Antibody (NB100-86983) (0)

There are no publications for PUS1 Antibody (NB100-86983).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PUS1 Antibody (NB100-86983) (0)

There are no reviews for PUS1 Antibody (NB100-86983). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PUS1 Antibody (NB100-86983) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

PUS1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

Additional PUS1 Products

Bioinformatics Tool for PUS1 Antibody (NB100-86983)

Discover related pathways, diseases and genes to PUS1 Antibody (NB100-86983). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PUS1 Antibody (NB100-86983)

Discover more about diseases related to PUS1 Antibody (NB100-86983).
 

Pathways for PUS1 Antibody (NB100-86983)

View related products by pathway.

PTMs for PUS1 Antibody (NB100-86983)

Learn more about PTMs related to PUS1 Antibody (NB100-86983).
 

Research Areas for PUS1 Antibody (NB100-86983)

Find related products by research area.

Blogs on PUS1

There are no specific blogs for PUS1, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PUS1 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol PUS1
Entrez
Uniprot