PUS1 Antibody

Western Blot: PUS1 Antibody [NB100-86983] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-86983 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.

Immunohistochemistry: PUS1 Antibody [NB100-86983] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PUS1 used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PUS1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 377 and 427 of human pseudouridylate synthase 1 using the numbering given in entry NP_079491.2 (GeneID 80324).
• Specificity: This antibody detects both isoforms of PUS1: Isoform 1 (47.5 kDa) and Isoform 2 (44.4kDa).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PUS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections
• Positive Control:

  PUS1 Lysate (NBL1-14996)
  PUS1 Lysate (NBP2-66155)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PUS1 Antibody

• EC 5.4.99.12
• MGC11268
• mitochondrial tRNA pseudouridine synthase A
• MLASA1
• pseudouridylate synthase 1
• tRNA pseudouridine synthase A, mitochondrial
• tRNA pseudouridylate synthase I
• tRNA uridine isomerase I
• tRNA-uridine isomerase I

Background

PUS1 converts uridine into pseudouridine after the nucleotide has been incorporated into RNA. Pseudouridine may have a functional role in tRNAs and may assist in the peptidyl transfer reaction of rRNAs (Chen and Patton, 1999 [PubMed 10094309]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PUS1
• Entrez:
  • 80324(Human)
• Uniprot:
  • NP_079491.2(Human)