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PUS1 Antibody

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Western Blot: PUS1 Antibody [NB100-86983] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-86983 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.
Independent Antibodies: Western Blot: PUS1 Antibody [NB100-86983] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-86983 used for WB at 0.04 mcg/ml (A) and 1 ...read more
Immunohistochemistry: PUS1 Antibody [NB100-86983] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PUS1 used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

        

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PUS1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 377 and 427 of human pseudouridylate synthase 1 using the numbering given in entry NP_079491.2 (GeneID 80324).
Specificity
This antibody detects both isoforms of PUS1: Isoform 1 (47.5 kDa) and Isoform 2 (44.4kDa).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PUS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections
Control
PUS1 Lysate (NBL1-14996)
PUS1 Lysate (NBP2-66155)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PUS1 Antibody

  • EC 5.4.99.12
  • MGC11268
  • mitochondrial tRNA pseudouridine synthase A
  • MLASA1
  • pseudouridylate synthase 1
  • tRNA pseudouridine synthase A, mitochondrial
  • tRNA pseudouridylate synthase I
  • tRNA uridine isomerase I
  • tRNA-uridine isomerase I

Background

PUS1 converts uridine into pseudouridine after the nucleotide has been incorporated into RNA. Pseudouridine may have a functional role in tRNAs and may assist in the peptidyl transfer reaction of rRNAs (Chen and Patton, 1999 [PubMed 10094309]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

PUS1 Overexpression Lysate (Native)
PUS1 Knockout 293T Cell Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PUS1
Entrez
Uniprot