PU.1/Spi-1 Antibody

Images

 
Western Blot: PU.1/Spi-1 Antibody [NBP1-00135] - (0.03ug/ml) Analysis of DAUDI lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody [NBP1-00135] - (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: PU.1/Spi-1 Antibody [NBP1-00135] - Analysis of PU.1/Spi1 in p3xFLAG-PU.1 transfected 293T cells (1 million cells) using anti-PU.1/Spi1 antibody. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PU.1/Spi-1 Antibody Summary

Immunogen
Peptide with sequence C-DLYQRQTHEYY corresponding to internal region according to NP_001074016.1, NP_003111.2.
Epitope
internal region
Specificity
This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SPI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 40 kDa band observed in lysates of DAUDI and K562 is reported in scientific literature (PMID: 10867017).
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-00135 in the following applications:

Publications
Read Publications using NBP1-00135.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PU.1/Spi-1 Antibody

  • hematopoietic transcription factor PU.1
  • OF
  • PU.1
  • SFPI1
  • SFPI131 kDa transforming protein
  • SPI-1 proto-oncogene
  • SPI1
  • Spi-1
  • SPI-A
  • spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein
  • transcription factor PU.1

Background

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PU.1/Spi-1 Antibody (NBP1-00135)(2)

Reviews for PU.1/Spi-1 Antibody (NBP1-00135) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: Flow, WB.

Reviews using NBP1-00135:
Filter by Applications
Flow
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry PU.1/Spi-1 NBP1-00135
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reviewed by:
Anonymous
Flow Human 10/16/2014
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Summary

ApplicationFlow Cytometry
Sample TestedControl 293T cell and cells transfected with p3XFLAG-PU.1
SpeciesHuman

Blocking

Blocking Details10% FCS for 1 hr at 4 degree

Primary Anitbody

Dilution Ratio1:100

Details

Detection Notes10,000 events detected on a FACSAria flow cytometer
Fixation DetailsFixed cells in 0.01% formaldehyde (5 min) at room temp
Permeabilization Details0.5% Triton X-100
Wash DescriptionWashed thrice (5 min each) with PBS
Western Blot PU.1/Spi-1 NBP1-00135
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reviewed by:
Anonymous
WB Human 10/16/2014
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Summary

ApplicationWestern Blot
Sample Tested293T lysate
SpeciesHuman

Blocking

Blocking Details5% BSA in PBS

Primary Anitbody

Dilution Ratio1:2000 diluted in PBS with triton X-100, blot was incubated at room temperature on a rocker

Secondary Antibody

Secondary Descriptionanti-Goat IgG HRP conjugate (Jackson Labs)
Secondary Manufacturer Cat#305-001-003
Secondary Concentration1:10,000

Details

Detection NotesProbed using Visualizer Western Blot Detection Kit (Millipore) and captured on X-ray films, 30 sec exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PU.1/Spi-1 Antibody (NBP1-00135) (0)

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Secondary Antibodies

 

Isotype Controls

Additional PU.1/Spi-1 Products

Bioinformatics Tool for PU.1/Spi-1 Antibody (NBP1-00135)

Discover related pathways, diseases and genes to PU.1/Spi-1 Antibody (NBP1-00135). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PU.1/Spi-1 Antibody (NBP1-00135)

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Recent Reviews

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5
1
4
0
3
1
2
0
1
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Anonymous
10/16/2014
Application: Flow
Species: Human

Anonymous
10/16/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol SPI1
Entrez
Uniprot