PU.1/Spi-1 Antibody (rPU1/2146)

Formalin-fixed, paraffin-embedded human lymph node stained with PU.1/Spi-1 Antibody (rPU1/2146).

Formalin-fixed, paraffin-embedded human lymph node stained with PU.1/Spi-1 Antibody (rPU1/2146).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: rPU1/2146
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PU.1/Spi-1 Antibody (rPU1/2146) Summary

• Description: Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
  
  Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa 16-170) of human PU.1 protein (exact sequence is proprietary) (Uniprot: P17947)
• Localization: Nucleus
• Source: CHO
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPI1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for PU.1/Spi-1 Antibody (rPU1/2146)

• hematopoietic transcription factor PU.1
• OF
• PU.1
• SFPI1
• SFPI131 kDa transforming protein
• SPI-1 proto-oncogene
• SPI1
• Spi-1
• SPI-A
• spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein
• transcription factor PU.1

Background

PU.1/Spi1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPI1