PU.1/Spi-1 Antibody (PU1/2146)

Images

 
Western Blot: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Analysis of THP-1 cell lysate using PU.1 Mouse Monoclonal Antibody (PU1/2146).
Immunocytochemistry/ Immunofluorescence: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU.1 with PU.1/Spi-1 Antibody (PU1/2146) followed by Goat anti-Mouse ...read more
Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Spleen stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).
Flow Cytometry: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Flow Cytometric Analysis of PFA-fixed Ramos cells. PU.1/Spi-1 Antibody (PU1/2146) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).
Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).
Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Formalin-fixed, paraffin-embedded human Lymph Node stained with PU.1 Mouse Monoclonal Antibody (PU1/2146).
Protein Array: PU.1/Spi-1 Antibody (PU1/2146) [NBP2-75738] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PU.1/Spi-1 Antibody (PU1/2146)Z- and S- Score: The Z-score represents ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, PA
Clone
PU1/2146
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

PU.1/Spi-1 Antibody (PU1/2146) Summary

Immunogen
Recombinant fragment (around aa 16-170) of human PU.1/Spi-1 protein (Exact sequence is proprietary)
Localization
Nuclear
Specificity
PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SPI1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot : 0.5-1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-75766)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for PU.1/Spi-1 Antibody (PU1/2146)

  • hematopoietic transcription factor PU.1
  • OF
  • PU.1
  • SFPI1
  • SFPI131 kDa transforming protein
  • SPI-1 proto-oncogene
  • SPI1
  • Spi-1
  • SPI-A
  • spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein
  • transcription factor PU.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics Tool for PU.1/Spi-1 Antibody (NBP2-75738)

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Bioinformatics

Gene Symbol SPI1
Uniprot