PTS Antibody (2B2)

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Western Blot: PTS Antibody (AT2B2) [NBP1-74035] Mouse kidney lysates (40ug) and recombinant protein were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PTS (1:500). Proteins were ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA
Clone
2B2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

PTS Antibody (2B2) Summary

Immunogen
Recombinant human PTS (1-145aa) purified from E. coli
Specificity
Anti-human PTS mAb, clone 2B2, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PTS protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTS
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
Application Notes
The antibody has been tested by ELISA, Western blot to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 1000. Recommended starting dilution is 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for PTS Antibody (2B2)

  • 6-pyruvoyl tetrahydrobiopterin synthase
  • 6-pyruvoyltetrahydropterin synthase
  • EC 4.2.3.12
  • FLJ97081
  • PTPSPTP synthase

Background

PTS or PTPS (6-Pyruvoyl tetrahydroprotein synthase) is involved in the biosynthesis of tetahydrobiopterin (BH4), the cofactor of several enzymes such as the nitric oxide synthase and the aromatic amino acid hdroxylases. Inherited deficiency of PTS is the most frequent cause of hyperphenylalninemia (HPA) characterized by depletion of the biogenic amine neutotransmitters, dopamine and serotonin, and is accompanied by severe progressive mental retardation. PTS was showed a homohexameric enzyme composed of a dimer or trimers in the crystallography and several experiments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PTS
Uniprot