PTRH2 Antibody

Images

 
Western Blot: PTRH2 Antibody [NBP1-76644] - Western blot analysis of PTRH2 in Daudi cell lysate with PTRH2 antibody using IMG-5425 at (A) 1, (B) 2, and (C) 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: PTRH2 Antibody [NBP1-76644] - Immunofluorescence of Bit1 in Human Small Intestine cells with Bit1 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: PTRH2 Antibody [NBP1-76644] - Human small intestine tissue with PTRH2 antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PTRH2 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide from near the center of human Bit1. The immunogen is located within amino acids 80 - 130 of Bit1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTRH2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Bit1 antibody can be used for the detection of Bit1 by Western blot at 1 - 2 mg/ml.
Positive Control
PTRH2 Lysate (NBP2-65982)
Control Peptide
PTRH2 Peptide (NBP1-76644PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PTRH2 Antibody

  • Bcl-2 inhibitor of transcription 1
  • BIT1CGI-147
  • EC 3.1.1.29
  • mitochondrial
  • peptidyl-tRNA hydrolase 2
  • PTH2FLJ32471

Background

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PTRH2
Uniprot