PTP rho/PTPRT Antibody

Western Blot: PTP rho/PTPRT Antibody [NB100-1459] - Analysis of PTPRT in human brain lysate (RIPA buffer, 35 ug total protein per lane) using this antibody at 0.3 ug/ml.

Product Discontinued

PTP rho/PTPRT Antibody Summary

• Immunogen: Peptide with sequence CNTWEKPMLDQAV corresponding to internal region according to NP_573400.2, NP_008981.3.
• Specificity: This antibody is expected to recognise both reported isoforms. Epitope corresponding to aa 58-69 of human PTPRT protein.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTPRT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 150 kDa band observed in human brain lysates (calculated MW of 164 kDa band according to NP_573400.2 and 163 kDa band according to NP_008981.3).
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTP rho/PTPRT Antibody

• EC 3.1.3
• EC 3.1.3.48
• KIAA0283RPTPrho
• protein tyrosine phosphatase, receptor type, T
• PTP rho
• PTPRT
• receptor protein tyrosine phosphatase
• Receptor-type tyrosine-protein phosphatase rho
• receptor-type tyrosine-protein phosphatase T
• RPTP-rho
• R-PTP-T

Background

The protein is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP is a large protein that possesses a PTP domain at C-terminus, and multiple noncatalytic domains, which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains, and a leucine zipper motif. This PTP was found to interact with, and dephosphorylate Fas receptor, as well as I-kappa B-alpha through the PDZ domains, which suggested its role in Fas mediated programmed cell death. This PTP was also shown to interact with GTPase-activating protein, and thus may function as a regulator of Rho signaling pathway. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTP rho/PTPRT Antibody (NB100-1459)(1)

Publication using NB100-1459

• Wang Z, Shen D, Parsons DW et al. Mutational analysis of the tyrosine phosphatome in colorectal cancers. Science 2004-05-21 [PMID: 15155950]

Bioinformatics

• Gene Symbol: PTPRT
• Entrez:
  • 11122(Human)
• Uniprot:
  • NP_008981(Human)