PTCHD1 Antibody

Western Blot: PTCHD1 Antibody [NBP1-52108] - Western blot analysis of PTCHD1 in Human Cerebellum lysate (35ug protein in RIPA buffer) using NBP1-52108 at 0.5ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PTCHD1 Antibody Summary

• Description: Novus Biologicals Goat PTCHD1 Antibody (NBP1-52108) is a polyclonal antibody validated for use in WB and ELISA. Anti-PTCHD1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DFQKTSRVSER corresponding to internal region according to NP_775766.2.
• Epitope: C-DFQKTSRVSER
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PTCHD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 100 kDa band observed in human brain (cerebellum) lysates (calculated MW of 101 kDa band according to NP_775766.2). An additional band of unknown identity was also consistently observed at 50 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PTCHD1 Antibody

• FLJ30296
• MGC149798
• patched domain containing 1
• patched domain-containing protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PTCHD1 Antibody (NBP1-52108)(4)

Publications using NBP1-52108

• Pinto D, Pagnamenta AT, Klei L et al. Functional impact of global rare copy number variation in autism spectrum disorders. Nature 2010-07-15 [PMID: 20531469]
• Farley K, Forbes C, Shaw N et al. CRISPR/Cas9 generated PTCHD1 2489T>G stem cells recapitulate patient phenotype when undergoing neural induction Research Square 2023-05-16 (WB, Human)
  
  Details:
  WB 1:500
• Farley K, Forbes C, Shaw N et al. CRISPR/Cas9 generated PTCHD1 2489T>G stem cells recapitulate patient phenotype when undergoing neural induction Research Square 2023-05-16 (WB, Human)
  
  Details:
  WB 1:500
• Farley KO, Forbes CA, Shaw NC et al. CRISPR/Cas9 generated PTCHD1 2489T>G stem cells recapitulate patient phenotype when undergoing neural induction HGG advances 2023-11-24 [PMID: 38007613] (WB, Human)
  
  Details:
  1:500 dilution

Bioinformatics

• Gene Symbol: PTCHD1
• Entrez:
  • 139411(Human)
  • 211612(Mouse)
  • 317517(Rat)
• Uniprot:
  • NP_775766.2(Human)