Human PSP/BPIFA2 - High Sensitivity ELISA Kit (Colorimetric)

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Standard Curve Range: 31.25 - 2000 pg/mL (example only; lot dependent)
• Sensitivity: 11.6 pg/mL (example only; lot dependent)

Human PSP/BPIFA2 - High Sensitivity ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 31.25 - 2000 pg/mL (example only; lot dependent)
• Sensitivity: 11.6 pg/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: BPIFA2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human PSP/BPIFA2 - High Sensitivity ELISA Kit (Colorimetric)

• bA49G10.1
• BPIFA2
• C20orf70
• chromosome 20 open reading frame 70
• Parotid secretory protein
• PSP
• short palate, lung and nasal epithelium carcinoma associated 2
• short palate, lung and nasal epithelium carcinoma-associated protein 2
• SPLUNC2

Background

Parotid secretory protein (PSP; also short palate, lung, nasal epithelium carcinoma-associated protein 2 or SPLUNC2 and C20orf70) is a 36 - 40 kDa secreted glycoprotein and member of the Bactericidal permeability-increasing (BPI)/Lipopolysaccharide-binding protein (LBP)/PLUNC superfamily and PLUNC family of proteins (1). Human PSP is synthesized as a 249 amino acid (aa) precursor that contains an 18 aa signal sequence and a 231 aa mature chain. The mature chain contains one BPI/LBS/PLUNC domain (aa 62 - 220) and two potential sites for N-linked glycosylation. Mature human PSP is 31% and 26% aa identical to mature mouse and rat PSP, respectively. Human PSP is expressed in the parotid and submandibular glands in ductal epithelial cells and acinar cells (1 - 2). PSP has been shown to exhibit bacteristatic and bactericidal effects on Pseudomonas aerginosa (1). In addition, PSP-derived peptides inhibit the binding of endotoxin to LBP and inhibit the endotoxin-stimulated secretion of tumor necrosis factor alpha from macrophages (1, 3). These findings suggest that PSP peptides can serve as templates for the design of novel anti-inflammatory peptides (3). One study showed that peptide GL-13 induced bacterial matting, suggesting passive agglutination of bacteria (4). GL-13 was shown to agglutinate Gram negative bacteria P. aerginosa and Aggregatibacter (Actinobacillus) actinomycetemcomitans, Gram positive Streptococcus gordonii and uncoated sheep erythrocytes (4). The agglutination leads to increased clearance by host phagocytic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for PSP/BPIFA2 ELISA Kit (NBP3-52332). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: BPIFA2