PSMC6 Antibody - BSA Free

Western Blot: PSMC6 Antibody [NBP2-19985] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: PSMC6 Antibody [NBP2-19985] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: PSMC6 Antibody [NBP2-19985] - Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using antibody at 1:500 dilution.

Western Blot: PSMC6 Antibody [NBP2-19985] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PSMC6 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PSMC6 Antibody - BSA Free (NBP2-19985) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PSMC6. The exact sequence is proprietary.
• Localization: Cytoplasm (By similarity); Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMC6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PSMC6 Antibody - BSA Free

• 26S protease regulatory subunit 10B
• 26S protease regulatory subunit S10B
• 26S proteasome AAA-ATPase subunit RPT4
• CADP44
• conserved ATPase domain protein 44
• MGC12520
• p42
• P44
• proteasome (prosome, macropain) 26S subunit, ATPase, 6
• Proteasome 26S subunit ATPase 6
• Proteasome subunit p42
• SUG2

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PSMC6
• Uniprot:
  • P62333(Human)