PSMC3IP Overexpression Lysate

Images

 
Western Blot: PSMC3IP Overexpression Lysate (Adult Normal) [NBL1-14889] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PSMC3IP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PSMC3IP Overexpression Lysate Summary

Description
Transient overexpression lysate of PSMC3 interacting protein (PSMC3IP), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC201045 and based on accession number NM_013290. The protein contains a C-MYC/DDK Tag.
Gene
PSMC3IP

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
23.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PSMC3IP Overexpression Lysate

  • DBD-interacting
  • GT198 alternative
  • GT198
  • homologous-pairing protein 2 homolog
  • HOP2
  • HUMGT198A
  • Nuclear receptor coactivator GT198
  • Proteasome 26S ATPase subunit 3-interacting protein
  • PSMC3 interacting protein
  • Tat-binding protein 1-interacting protein
  • TBP-1 interacting protein
  • TBP-1-interacting protein
  • TBPIPPSMC3-interacting protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Other Available Formats

NBL1-14889

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Bioinformatics

Gene Symbol PSMC3IP