PSMA4 Antibody (OTI4B8) - Azide and BSA Free

Western Blot: PSMA4 Antibody (OTI4B8) - Azide and BSA Free [NBP2-73690] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA4.

Immunohistochemistry: PSMA4 Antibody (OTI4B8) - Azide and BSA Free [NBP2-73690] - Staining of paraffin-embedded Human pancreas tissue using anti-PSMA4 mouse monoclonal antibody.

Western Blot: PSMA4 Antibody (OTI4B8) - Azide and BSA Free [NBP2-73690] - Analysis of extracts (35ug) from 9 different cell lines by using anti-PSMA4 monoclonal antibody.

Immunohistochemistry: PSMA4 Antibody (OTI4B8) - Azide and BSA Free [NBP2-73690] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA4 mouse monoclonal antibody.

Immunohistochemistry: PSMA4 Antibody (OTI4B8) - Azide and BSA Free [NBP2-73690] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMA4 mouse monoclonal antibody.

Immunohistochemistry: PSMA4 Antibody (OTI4B8) - Azide and BSA Free [NBP2-73690] - Staining of paraffin-embedded Human endometrium tissue using anti-PSMA4 mouse monoclonal antibody.

Immunohistochemistry: PSMA4 Antibody (OTI4B8) - Azide and BSA Free [NBP2-73690] - Staining of paraffin-embedded Human Kidney tissue using anti-PSMA4 mouse monoclonal antibody.

Immunohistochemistry: PSMA4 Antibody (OTI4B8) - Azide and BSA Free [NBP2-73690] - Staining of paraffin-embedded Human prostate tissue using anti-PSMA4 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Pm, Pm
• Applications: WB, IHC
• Clone: OTI4B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PSMA4 Antibody (OTI4B8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PSMA4 Antibody (OTI4B8) - Azide and BSA Free (NBP2-01608) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PSMA4(NP_002780) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSMA4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 29.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PSMA4 Antibody (OTI4B8) - Azide and BSA Free

• HC9
• HC9EC 3.4.25.1
• HsT17706
• Macropain subunit C9
• MGC12467
• MGC24813
• Multicatalytic endopeptidase complex subunit C9
• proteasome (prosome, macropain) subunit, alpha type, 4
• Proteasome component C9
• proteasome subunit alpha type-4
• proteasome subunit HC9
• Proteasome subunit L
• PSC9
• PSC9MGC111191
• PSMA4

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PSMA4