PSF2 Antibody

Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-GINS2 )

Product Discontinued

PSF2 Antibody Summary

• Immunogen: The immunogen for this antibody was a sequence from 1a.a. to 185a.a. from PSF2(a 185a.a. long protein)
• Localization: Nuclear
• Specificity: PSF2
• Clonality: Polyclonal
• Host: Chicken
• Gene: GINS2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000. Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
• Control:

  PSF2 Overexpression Lysate

Reactivity Notes

This antibody is reactive against Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for PSF2 Antibody

• DNA replication complex GINS protein PSF2
• GINS complex subunit 2 (Psf2 homolog)
• GINS complex subunit 2
• HSPC037
• PSF2Pfs2

Background

The GINS complex is composed of four subunits, encoded by SLD5, PSF1, PSF2, and PSF3. In S. cerevisiae, it was first identified by genetic and biochemical methods to determine factors interacting with Sld5p. Genetic interactions between these four genes also suggest that they act together. The GINS complex was independently isolated in a large scale screen for cell cycle defects. A similar complex is found in Xenopus and has a ring-like structure. In yeast, all four genes are essential and cells defective in SLD5, PSF1, or PSF2 are impaired in their ability to replicate DNA. The complex localizes to origins of DNA replication and Sld5p was previously implicated as functioning in DNA replication due to its genetic interaction with DPB11. Additional genetic and biochemical interactions of the GINS complex with Dpb11p, Dpb2p, and Sld3p suggest that it functions in some way at the replication fork during DNA synthesis (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GINS2
• Entrez:
  • 51659(Human)
• Uniprot:
  • Q53G08(Human)