PRR5 Antibody

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human PRR5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PRR5 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PRR5 Antibody Summary

• Immunogen: E. coli-derived recombinant human PRR5
  Tyr126-Ala259
  Accession # P85299
• Specificity: Detects endogenous human PRR5 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRR5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRR5 Antibody

• FLJ20185
• FLJ20185k
• PP610
• proline rich 5 (renal)
• protein observed with Rictor-1
• Protor-1
• PRR5

Background

PRR5 (Pro-rich protein 5; also Protor-1) is a 42‑46 kDa member of the Protor family of proteins. It is widely expressed, and forms part of mTORC2, an enzyme complex that regulates Atk and PKC alpha . At present, it appears PRR5 promotes mTORC2 activity. Human PRR5 is 388 amino acids (aa) in length. It possesses two RICTOR-interaction sites (aa 10‑95 and 188‑218) and a C-terminal Pro-rich region. There are multiple splice forms of PRR5. Either individually, or in-common, there is an alternate start site at Met10 ( beta isoform) and Met96 ( gamma isoform), a deletion of aa 45‑71, 161‑388, and 236‑388, a 17 aa insertion after Gly108, and a six aa substitution for aa 1‑107. Over aa 26‑259 of the 41 kDa beta isoform (NP_056181), there is 95% aa identity between human and mouse PRR5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRR5
• Uniprot:
  • P85299()