PRPF4B Antibody (3E10)

Immunocytochemistry/Immunofluorescence: PRPF4B Antibody (3E10) [H00008899-M01] - Analysis of monoclonal antibody to PRPF4B on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: PRPF4B Antibody (3E10) [H00008899-M01] - Detection limit for recombinant GST tagged PRPF4B is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PRPF4B Antibody (3E10) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PRPF4B (NP_003904.2, 898 a.a. ~ 1005 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LKLAMDLKGKMPNKMIRKGVFKDQHFDQNLNFMYIEVDKVTEREKVTVMSTINPTKDLLADLIGCQRLPEDQRKKVHQLKDLLDQILMLDPAKRISINQALQHAFIQE
• Specificity: PRPF4B - PRP4 pre-mRNA processing factor 4 homolog B (yeast) (3E10)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRPF4B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PRPF4B Antibody (3E10)

• dJ1013A10.1 (PRP4 protein kinase homolog)
• dJ1013A10.1
• KIAA0536EC 2.7.11.1
• PR4H
• protein-serine/threonine kinase
• PRP4 kinase
• PRP4 pre-mRNA processing factor 4 homolog B (yeast)
• PRP4 pre-mRNA-processing factor 4 homolog
• PRP4H
• PRP4K
• PRP4Prp4
• serine/threonine-protein kinase PRP4 homolog

Background

Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRPF4B Antibody (H00008899-M01)(1)

Publication using H00008899-M01

• Fang CL, Uen YH, Chen HK et al. Loss of cyclin-dependent kinase-like 2 predicts poor prognosis in gastric cancer, and its overexpression suppresses cells growth and invasion. Cancer Med 2018-05-23 [PMID: 29790675]

Bioinformatics

• Gene Symbol: PRPF4B
• Entrez:
  • 8899(Human)
• Uniprot:
  • NP_003904(Human)