PRPF3 Antibody - BSA Free

Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114] - Detection of mouse hPrp3p by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-hPrp3p (NBP1-19114) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-hPrp3p used at a dilution of 1:100.

Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114] - FFPE section of mouse teratoma. Affinity purified rabbit anti-hPrp3p used at a dilution of 1:100.

Immunohistochemistry: PRPF3 Antibody [NBP1-19114] - Sample: FFPE section of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-hPrp3p used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114] - Detection of human hPrp3p by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-hPrp3p (NBP1-19114) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: PRPF3 Antibody [NBP1-19114] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-19114 used for IP at 3 mcg/mg lysate. hPrp3p was also immunoprecipitated by NBP1-19115 which recognizes a downstream epitope

• Reactivity: Hu, Mu
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PRPF3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PRPF3 Antibody - BSA Free (NBP1-19114) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human U4/U6 small nuclear ribonucleoprotein Prp3 using the numbering given in entry NP_004689.1 (GeneID 9129).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRPF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: It is not recommended for western analysis.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRPF3 Antibody - BSA Free

• hPrp3
• HPRP3P
• HPRP3retinitis pigmentosa 18 (autosomal dominant)
• Pre-mRNA-splicing factor 3
• PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae)
• PRP3 pre-mRNA processing factor 3 homolog (yeast)
• PRP3
• Prp3p
• RP18
• U4/U6 small nuclear ribonucleoprotein Prp3
• U4/U6 snRNP 90 kDa protein
• U4/U6-associated RNA splicing factor

Background

The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRPF3
• Entrez:
  • 9129(Human)
• Uniprot:
  • NP_004689.1(Human)