Human Proteinase 3/Myeloblastin/PRTN3 ELISA Kit (Colorimetric)

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Samples were spiked with high concentrations of Human Proteinase 3/Myeloblastin/PRTN3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Proteinase 3/Myeloblastin/PRTN3 ELISA Kit (Colorimetric) [NBP3-18227] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

Human Proteinase 3/Myeloblastin/PRTN3 ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 5.88
Intra-Assay
%CV < 4.48
Spike Recovery
93-112 %
Kit Type
ELISA Kit (Colorimetric)
Gene
PRTN3

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent - 1 Vial
  2. Concentrated Biotinylated Detection Ab (100×) - 120 uL
  3. Concentrated HRP Conjugate (100×) - 120 uL
  4. Concentrated Wash Buffer (25×) - 30 mL
  5. HRP Conjugate Diluent - 1 Vial
  6. Micro ELISA Plate(Dismountable)
  7. Plate Sealer - 5 Pieces
  8. Reference Standard - 2 Vials
  9. Reference Standard & Sample Diluent - 20 mL
  10. Stop Solution - 1 Vial
  11. Substrate Reagent - 1 Vial

Alternate Names for Human Proteinase 3/Myeloblastin/PRTN3 ELISA Kit (Colorimetric)

  • ACPA
  • AGP7
  • AGP7PR3
  • azurophil granule protein 7
  • C-ANCA
  • EC 3.4.21
  • EC 3.4.21.76
  • Leukocyte proteinase 3
  • MBN
  • MBT
  • Myeloblastin
  • Neutrophil proteinase 4
  • NP4
  • NP-4
  • P29C-ANCA antigen
  • PR-3CANCA
  • Proteinase 3
  • PRTN3
  • serine proteinase, neutrophil
  • Wegener autoantigen
  • Wegener granulomatosis autoantigen

Background

Myeloblastin (PR3) is a polymorphonuclear leukocyte serine protease which degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. PR3 plays a role in neutrophil response to inflammation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Proteinase 3/Myeloblastin/PRTN3 ELISA Kit (NBP3-18227). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol PRTN3