Protein Kinase A Regulatory Subunit II alpha Antibody (2394D) [Unconjugated]

HAF008). A specific band was detected for PKA RIIa at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF008). A specific band was detected for PKA RIIa at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse PKA RIIa Monoclonal Antibody (Catalog # MAB8000) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PKA RIIa at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse PKA RIIa Monoclonal Antibody (Catalog # MAB8000) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="PKA RIIa was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Rabbit Anti-Human/Mouse PKA RIIa Monoclonal Antibody (Catalog # MAB8000) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

PKA RIIa was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Rabbit Anti-Human/Mouse PKA RIIa Monoclonal Antibody (Catalog # MAB8000) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." > VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." title="PKA RIIa was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Rabbit Anti-Human/Mouse PKA RIIa Monoclonal Antibody (Catalog # MAB8000) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." />

PKA RIIa was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Rabbit Anti-Human/Mouse PKA RIIa Monoclonal Antibody (Catalog # MAB8000) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

• Applications: WB, IHC, ICC/IF
• Clone: 2394D
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Protein Kinase A Regulatory Subunit II alpha Antibody (2394D) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide containing Human/Mouse PKA RII alpha
  Accession # P13861
• Specificity: Detects human PKA RII alpha in direct ELISAs. Detects human and mouse PKA RII alpha in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 0.3-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protein Kinase A Regulatory Subunit II alpha Antibody (2394D) [Unconjugated]

• cAMP-dependent protein kinase regulatory subunit RII alpha
• cAMP-dependent protein kinase type II-alpha regulatory subunit
• PKA RII alpha
• PKR2
• PRKAR2
• PRKAR2A
• protein kinase A, RII-alpha subunit
• protein kinase, cAMP-dependent, regulatory subunit type II alpha

Background

PRKAR2 is a regulatory subunit of the cAMP-dependent protein kinases involved in CAMP signaling in cells.  Four types of regulatory chains are found and their expression varies among tissues.  In some cases expression is constitutive and in other cases it is inducible.  PRKAR2 seems to be involved in both endosome-to-Golgi and Golgi-to-ER transport.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Protein Kinase A Regulatory Subunit II alpha Antibody (MAB8000)(1)

Publication using MAB8000

• GF Jang, JS Crabb, B Hu, B Willard, H Kalirai, AD Singh, SE Coupland, JW Crabb Proteomics of Primary Uveal Melanoma: Insights into Metastasis and Protein Biomarkers Cancers, 2021-07-14;13(14):. 2021-07-14 [PMID: 34298739] (Human)

Bioinformatics

• Uniprot:
  • P13861()