Protein Kinase A regulatory subunit I alpha Antibody

Western Blot: Protein Kinase A regulatory subunit I alpha Antibody [H00005573-B01P] - Analysis of PRKAR1A expression in transfected 293T cell line by PRKAR1A polyclonal antibody. Lane 1: PRKAR1A transfected lysate(41.91 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

Protein Kinase A regulatory subunit I alpha Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: PRKAR1A (NP_002725.1, 1 a.a. - 381 a.a.) full-length human protein. MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQGETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV
• Specificity: PRKAR1A - protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: PRKAR1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Protein Kinase A regulatory subunit I alpha Antibody

• cAMP-dependent protein kinase regulatory subunit RIalpha
• cAMP-dependent protein kinase type I-alpha regulatory chain
• cAMP-dependent protein kinase type I-alpha regulatory subunit
• CAR
• CNC
• CNC1
• MGC17251
• PKA R1 alpha
• PKA1
• PKR1
• PPNAD1
• PRKAR1
• PRKAR1A
• Protein Kinase A regulatory subunit I alpha
• protein kinase A type 1a regulatory subunit
• protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specificextinguisher 1)
• Tissue-specific extinguisher 1
• TSE1
• TSE1DKFZp779L0468

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Protein Kinase A regulatory subunit I alpha Antibody (H00005573-B01P)(1)

Publication using H00005573-B01P

• Uys GM, Ramburan A, Loos B et al. Myomegalin is a novel A-kinase anchoring protein involved in the phosphorylation of cardiac myosin binding protein C. BMC Cell Biol 2011-05-10 [PMID: 21569246]

Bioinformatics

• Gene Symbol: PRKAR1A
• Entrez:
  • 5573(Human)
• Uniprot:
  • NP_002725.1(Human)