Proteasome subunit beta type 4 Antibody

Images

 
Western Blot: Proteasome subunit beta type 4 Antibody [NBP1-52080] - (0.3ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Proteasome subunit beta type 4 Antibody [NBP1-52080] - (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Proteasome subunit beta type 4 Antibody Summary

Immunogen
Peptide with sequence C-NRFQIATVTE corresponding to internal region according to NP_002787.2.
Epitope
C-NRFQIATVTE
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PSMB4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 25-26 kDa kDa band observed in HEK293, HeLa, and K562 lysates and in mouse and rat liver lysates (calculated MW of 29.1 kDa band according to NP_002787.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
Publications
Read Publication using NBP1-52080.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine, Rabbit.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Proteasome subunit beta type 4 Antibody

  • EC 3.4.25.1
  • HN3
  • hsBPROS26
  • HsN3
  • Macropain beta chain
  • Multicatalytic endopeptidase complex beta chain
  • PROS26PROS-26
  • proteasome (prosome, macropain) subunit, beta type, 4
  • Proteasome beta chain
  • Proteasome chain 3
  • proteasome subunit beta type-4
  • proteasome subunit HsN3
  • proteasome subunit, beta type, 4,26 kDa prosomal protein

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Proteasome subunit beta type 4 Antibody (NBP1-52080)(1)

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PSMB4
Entrez
Uniprot