Proteasome subunit beta type 4 Antibody

Western Blot: Proteasome subunit beta type 4 Antibody [NBP1-52080] - Staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: Proteasome subunit beta type 4 Antibody [NBP1-52080] - (0.3ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Proteasome subunit beta type 4 Antibody Summary

• Immunogen: Peptide with sequence C-NRFQIATVTE corresponding to internal region according to NP_002787.2.
• Epitope: C-NRFQIATVTE
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PSMB4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 25-26 kDa kDa band observed in HEK293, HeLa, and K562 lysates and in mouse and rat liver lysates (calculated MW of 29.1 kDa band according to NP_002787.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Proteasome subunit beta type 4 Antibody

• EC 3.4.25.1
• HN3
• HsBPROS26
• HsN3
• Macropain beta chain
• Multicatalytic endopeptidase complex beta chain
• PROS26
• PROS-26
• PROS26PROS-26
• proteasome (prosome, macropain) subunit, beta type, 4
• Proteasome beta chain
• Proteasome chain 3
• Proteasome subunit beta type 4
• proteasome subunit beta type-4
• proteasome subunit HsN3
• proteasome subunit, beta type, 4,26 kDa prosomal protein
• PSMB4

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Proteasome subunit beta type 4 Antibody (NBP1-52080)(1)

Publication using NBP1-52080

• Yoshida T, Kato K, Yokoi K et al. Association of genetic variants with hemorrhagic stroke in Japanese individuals. Int J Mol Med 2010-04-01 [PMID: 20198315]

Bioinformatics

• Gene Symbol: PSMB4
• Entrez:
  • 5692(Human)
  • 19172(Mouse)
  • 58854(Rat)
• Uniprot:
  • NP_002787.2(Human)