Proteasome 20S beta2 Overexpression Lysate

Images

 
Western Blot: Proteasome 20S beta2 Overexpression Lysate (Adult Normal) [NBL1-14877] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Proteasome 20S beta2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Proteasome 20S beta2 Overexpression Lysate Summary

Description

Proteasome 20S beta2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC210452

Accession#: NM_002794

Protein Tag: C-MYC/DDK
Gene
PSMB2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
22.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Proteasome 20S beta2 Overexpression Lysate

  • EC 3.4.25.1
  • HC7-I
  • Macropain subunit C7-I
  • MGC104215
  • MGC126885
  • multicatalytic endopeptidase complex subunit C7-1
  • Multicatalytic endopeptidase complex subunit C7-I
  • proteasome (prosome, macropain) subunit, beta type, 2
  • Proteasome 20S beta2
  • proteasome beta 2 subunit
  • Proteasome component C7-I
  • proteasome subunit beta type-2
  • proteasome subunit, beta type, 2
  • PSMB2

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Proteasome 20S beta2 Lysate (NBL1-14877) (0)

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Bioinformatics

Gene Symbol PSMB2