Prosurfactant Protein C Overexpression Lysate

Images

 
Western Blot: Prosurfactant Protein C Overexpression Lysate (Adult Normal) [NBL1-15896] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Prosurfactant Protein C.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Prosurfactant Protein C Overexpression Lysate Summary

Description
Transient overexpression lysate of surfactant protein C (SFTPC) The lysate was created in HEK293T cells, using Plasmid ID RC203246 and based on accession number NM_003018. The protein contains a C-MYC/DDK Tag.
Gene
SFTPC

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
20.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Prosurfactant Protein C Overexpression Lysate

  • PSP-C
  • pulmonary surfactant-associated protein C
  • Pulmonary surfactant-associated proteolipid SPL(Val)
  • SFTP2
  • SFTP2pulmonary surfactant apoprotein-2 SP-C
  • SMDP2surfactant, pulmonary-associated protein C
  • SP-CSP5
  • surfactant protein C

Background

The SFTPC gene encodes pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. It is produced exclusively by type II alveolar epithelial cells in the lung. Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. Surfactant is composed of phospholipids and other surfactant-associated proteins (Clark and Clark, 2005 [PubMed 15927881]). See also SFTPA1 (MIM 178630), SFTPB (MIM 178640), and SFTPD (MIM 178635).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Other Available Formats

NBL1-15896

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Bioinformatics

Gene Symbol SFTPC