Proprotein Convertase 9/PCSK9 Antibody

Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NBP1-51923] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PCSK9 with C-terminal MYC tag probed with NBP1-51923 (0.5ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with NBP1-51923 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Proprotein Convertase 9/PCSK9 Antibody Summary

• Description: Novus Biologicals Goat Proprotein Convertase 9/PCSK9 Antibody (NBP1-51923) is a polyclonal antibody validated for use in WB and ELISA. Anti-Proprotein Convertase 9/PCSK9 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-PRYRADEYQPPD corresponding to internal region according to NP_777596.2.
• Epitope: C-PRYRADEYQPPD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PCSK9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.5 ug/ml
• Application Notes: WB: In transfected HEK293 transiently expressing full-length human PCSK9 (myc and DYKDDDDK tagged), a doublet of approx. 80-100 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same bands were observed using anti-myc tag antibody.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Proprotein Convertase 9/PCSK9 Antibody

• EC 3.4.21
• EC 3.4.21.111
• FH3
• FH3neural apoptosis regulated convertase 1
• FHCL3
• HCHOLA3
• hypercholesterolemia, autosomal dominant 3
• LDLCQ1
• NARC1
• NARC-1
• NARC-1convertase subtilisin/kexin type 9 preproprotein
• NARC1EC 3.4.21.-
• Neural apoptosis-regulated convertase 1
• PC9
• PCSK9
• Proprotein Convertase 9
• proprotein convertase subtilisin/kexin type 9
• Subtilisin/kexin-like protease PC9

Background

This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. The encoded protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. This protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a third form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Proprotein Convertase 9/PCSK9 Antibody (NBP1-51923)(1)

Publication using NBP1-51923

• Lalanne F, Lambert G, Amar MJ et al. Wild-type PCSK9 inhibits LDL clearance but does not affect apoB-containing lipoprotein production in mouse and cultured cells. J Lipid Res 2005-06-01 [PMID: 15741654]

Bioinformatics

• Gene Symbol: PCSK9
• Entrez:
  • 255738(Human)
• Uniprot:
  • NP_777596.2(Human)