Proprotein Convertase 9/PCSK9 Antibody [Unconjugated]

Western blot shows lysates of mouse liver tissue and rat embryonic cortical neuron. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Proprotein Convertase 9/PCSK9 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Proprotein Convertase 9/PCSK9 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Proprotein Convertase 9
  Ser156-Gln694
  Accession # Q80W65
• Specificity: Detects mouse and rat Proprotein Convertase 9/PCSK9 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PCSK9
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Proprotein Convertase 9/PCSK9 Antibody [Unconjugated]

• EC 3.4.21
• EC 3.4.21.111
• FH3
• FH3neural apoptosis regulated convertase 1
• FHCL3
• HCHOLA3
• hypercholesterolemia, autosomal dominant 3
• LDLCQ1
• NARC1
• NARC-1
• NARC-1convertase subtilisin/kexin type 9 preproprotein
• NARC1EC 3.4.21.-
• Neural apoptosis-regulated convertase 1
• PC9
• PCSK9
• Proprotein Convertase 9
• proprotein convertase subtilisin/kexin type 9
• Subtilisin/kexin-like protease PC9

Background

PC9 (Proprotein convertase subtilisin type 9; also PCSK9 and NARC-1) is a 70‑74 kDa, secreted glycoprotein member of the proteinase K subfamily of subtilases. PC9 functions as a protease that cleaves proproteins at non-basic residues. It also binds to, and promotes, the degradation of LDLR, VLDLR and ApoER2. The mouse PC9 precursor is 694 amino acid (aa) in length, and contains a signal sequence, a 14 kDa, 121 aa prodomain (aa 35‑155), and a 56‑58 kDa, 539 aa mature region (aa 156‑694). The 58 kDa mature region arises from autocatalysis in the ER, and contains a subtilisin-like catalytic domain (aa 166‑432), a modified P-domain (aa 433‑529) and a C-terminal Cys-rich region (aa 530‑694). The prosegment and mature regions remain noncovalently associated. There is one additional PC9 isoform that shows an alternative start site 13 aa upstream of the standard site coupled to a deletion of aa 18‑20. Over aa 156‑694, mouse PC9 shares 93% and 79% aa identity with rat and human PC9, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Proprotein Convertase 9/PCSK9 Antibody (AF3985)(20)

Publications using AF3985

• Wang, Y;Tinsley, B;Spolitu, S;Zadroga, JA;Agarwal, H;Sarecha, AK;Ozcan, L; Geranylgeranyl Isoprenoids and Hepatic Rap1a Regulate Basal and Statin-Induced Expression of PCSK9 bioRxiv : the preprint server for biology 2023-10-23 [PMID: 37961667] (ICC, Mouse)
• D Pakalnišky, T Schönberge, B Strobel, B Stierstorf, T Lamla, M Schuler, M Lenter Rosa26-LSL-dCas9-VPR: a versatile mouse model for tissue specific and simultaneous activation of multiple genes for drug discovery Scientific Reports, 2022-11-10;12(1):19268. 2022-11-10 [PMID: 36357523] (Simple Western, Transgenic Mouse)
• CT Stomberski, NM Venetos, HL Zhou, Z Qian, BR Collison, SJ Field, RT Premont, JS Stamler A multienzyme S-nitrosylation cascade regulates cholesterol homeostasis Cell Reports, 2022-10-25;41(4):111538. 2022-10-25 [PMID: 36288700] (ICC, Western Blot, Mouse)
• Marit Hjorth, Atanaska Doncheva, Frode Norheim, Stine Marie Ulven, Kirsten Bjørklund Holven, Thomas Sæther et al. Consumption of salmon fishmeal increases hepatic cholesterol content in obese C57BL/6 J mice European Journal of Nutrition 2022-12-01 [PMID: 35788891] (Western Blot, Mouse)
• Marit Hjorth, Atanaska Doncheva, Frode Norheim, Stine Marie Ulven, Kirsten Bjørklund Holven, Thomas Sæther et al. Consumption of salmon fishmeal increases hepatic cholesterol content in obese C57BL/6 J mice European Journal of Nutrition 12/1/2022 [PMID: 35788891]
• Á Petersen-U, M Kremser, AK Rohlfing, T Castor, K Kolb, V Dicenta, F Emscherman, B Li, O Borst, D Rath, KAL Müller, MP Gawaz Platelet-Derived PCSK9 Is Associated with LDL Metabolism and Modulates Atherothrombotic Mechanisms in Coronary Artery Disease International Journal of Molecular Sciences, 2021-10-16;22(20):. 10/16/2021 [PMID: 34681838]
• EM Haberl, R Pohl, L Rein-Fisch, M Höring, S Krautbauer, G Liebisch, C Buechler Accumulation of cholesterol, triglycerides and ceramides in hepatocellular carcinomas of diethylnitrosamine injected mice Lipids in Health and Disease, 2021-10-10;20(1):135. 2021-10-10 [PMID: 34629057] (Western Blot, Mouse)
• T Rothgangl, MK Dennis, PJC Lin, R Oka, D Witzigmann, L Villiger, W Qi, M Hruzova, L Kissling, D Lenggenhag, C Borrelli, S Egli, N Frey, N Bakker, JA Walker, AP Kadina, DV Victorov, M Pacesa, S Kreutzer, Z Kontarakis, A Moor, M Jinek, D Weissman, M Stoffel, R van Boxtel, K Holden, N Pardi, B Thöny, J Häberle, YK Tam, SC Semple, G Schwank In vivo adenine base editing of PCSK9 in macaques reduces LDL cholesterol levels Nature Biotechnology, 2021-05-19;0(0):. 2021-05-19 [PMID: 34012094] (Western Blot, Mouse)
• X Liu, X Bao, M Hu, H Chang, M Jiao, J Cheng, L Xie, Q Huang, F Li, CY Li Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer Nature, 2020-11-11;0(0):. 2020-11-11 [PMID: 33177715] (In Vivo, Mouse)
• Annemarie Wolf, Hanna Sarah Kutsche, Rolf Schreckenberg, Martin Weber, Ling Li, Susanne Rohrbach et al. Autocrine effects of PCSK9 on cardiomyocytes Basic Research in Cardiology 12/1/2020 [PMID: 33169229]
• KC Krishnan, RR Floyd, S Sabir, DW Jayasekera, PV Leon-Mimil, AE Jones, AA Cortez, V Shravah, M Péterfy, L Stiles, S Canizales-, AS Divakaruni, A Huertas-Va, AJ Lusis Liver Pyruvate Kinase Promotes NAFLD/NASH in both Mice and Humans in a Sex-Specific Manner Cell Mol Gastroenterol Hepatol, 2020-09-14;0(0):. 2020-09-14 [PMID: 32942044] (Western Blot, Mouse)
• Stefano Spolitu, Haruka Okamoto, Wen Dai, John A. Zadroga, Erika S. Wittchen, Jesper Gromada et al. Hepatic Glucagon Signaling Regulates PCSK9 and Low-Density Lipoprotein Cholesterol Circulation Research 2019-01-04 [PMID: 30582457] (Western Blot, Transgenic Mouse)
• Stefano Spolitu, Haruka Okamoto, Wen Dai, John A. Zadroga, Erika S. Wittchen, Jesper Gromada et al. Hepatic Glucagon Signaling Regulates PCSK9 and Low-Density Lipoprotein Cholesterol Circulation Research 1/4/2019 [PMID: 30582457]
• Alireza Edraki, Aamir Mir, Raed Ibraheim, Ildar Gainetdinov, Yeonsoo Yoon, Chun-Qing Song et al. A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome Editing Molecular Cell 2/1/2019 [PMID: 30581144]
• Agnieszka Dettlaff-Pokora, Elzbieta Sucajtys-Szulc, Tomasz Sledzinski Up-regulation of PCSK9 gene expression and diminished level of LDL-receptor in rat liver as a potential cause of post-lipectomy hypercholesterolemia Molecular and Cellular Biochemistry 5/1/2019 [PMID: 30483910]
• Aimee E. Vozenilek, Cassidy M.R. Blackburn, Robert M. Schilke, Sunitha Chandran, Reneau Castore, Ronald L. Klein et al. AAV8-mediated overexpression of mPCSK9 in liver differs between male and female mice Atherosclerosis 11/1/2018 [PMID: 30253291]
• R Ibraheim, CQ Song, A Mir, N Amrani, W Xue, EJ Sontheimer All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo Genome Biol., 2018-09-19;19(1):137. 2018-09-19 [PMID: 30231914] (Western Blot, Mouse)
• K Kim, IJ Goldberg, MJ Graham, M Sundaram, E Bertaggia, SX Lee, L Qiang, RA Haeusler, D Metzger, P Chambon, Z Yao, HN Ginsberg, UB Pajvani ?-Secretase Inhibition Lowers Plasma Triglyceride-Rich Lipoproteins by Stabilizing the LDL Receptor Cell Metab., 2018-03-22;0(0):. 2018-03-22 [PMID: 29576536] (Western Blot, Mouse, Rat)
• C Gustafsen, D Olsen, J Vilstrup, S Lund, A Reinhardt, N Wellner, T Larsen, CBF Andersen, K Weyer, JP Li, PH Seeberger, S Thirup, P Madsen, S Glerup Heparan sulfate proteoglycans present PCSK9 to the LDL receptor Nat Commun, 2017-09-11;8(1):503. 2017-09-11 [PMID: 28894089] (Western Blot, Hamster, Human)
• Elzbieta Sucajtys-Szulc, Marek Szolkiewicz, Julian Swierczynski, Boleslaw Rutkowski Up-regulation of liver Pcsk9 gene expression as a possible cause of hypercholesterolemia in experimental chronic renal failure Molecular and Cellular Biochemistry 1/1/2016 [PMID: 26481479]

Reviews for Proprotein Convertase 9/PCSK9 Antibody (AF3985) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 12/19/2014

Summary

• Application: Western Blot
• Sample Tested: Primary mouse hepatocytes
• Species: Mouse

reviewed by: Verified Customer WB Mouse 12/19/2014

Summary

• Application: Western Blot
• Sample Tested: Liver homogenates sample
• Species: Mouse

Bioinformatics

• Gene Symbol: PCSK9
• Uniprot:
  • Q80W65()