Proprotein Convertase 9/PCSK9 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Proprotein Convertase 9/PCSK9 ...read more
Recombinant Human Proprotein Convertase 9/PCSK9 protein was serially diluted 2-fold and captured by Rat Anti-Human Proprotein Convertase 9/PCSK9 Monoclonal Antibody (Catalog # MAB38883) coated on a Clear ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Proprotein Convertase 9/PCSK9 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human PCSK9 isoform 1
Gln31-Gln692
Accession # Q8NBP7
Specificity
Detects human PCSK9 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse PCSK9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PCSK9
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • ELISA
  • Immunoprecipitation 25 ug/mL
Control
Proprotein Convertase 9/PCSK9 Knockout HeLa Cell Lysate
Reviewed Applications
Read 5 Reviews rated 3.8
using
AF3888 in the following applications:

Publications
Read Publications using
AF3888 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Proprotein Convertase 9/PCSK9 Antibody [Unconjugated]

  • EC 3.4.21
  • EC 3.4.21.111
  • FH3
  • FH3neural apoptosis regulated convertase 1
  • FHCL3
  • HCHOLA3
  • hypercholesterolemia, autosomal dominant 3
  • LDLCQ1
  • NARC1
  • NARC-1
  • NARC-1convertase subtilisin/kexin type 9 preproprotein
  • NARC1EC 3.4.21.-
  • Neural apoptosis-regulated convertase 1
  • PC9
  • PCSK9
  • Proprotein Convertase 9
  • proprotein convertase subtilisin/kexin type 9
  • Subtilisin/kexin-like protease PC9

Background

The human PCSK9 gene encodes Proprotein Convertase 9 (PC9), which is also known as Neural Apoptosis Regulated Convertase 1 (NARC1) (1). The deduced amino acid sequence of human PCSK9 consists of a signal peptide (residues 1-30), a pro peptide (residue 31-152), and a mature chain (residues 153-692) that contains a serine protease domain (residues 161-431) found in members of the furin/PC family. PCSK9 protease activity may be limited, since it has only been demonstrated through its own autocatalytic processing (2). After the autocleavage in the ER, the pro domain and mature chain exit the cell together through non-covalent interactions (3). PCSK9 is a key regulator of LDL-cholesterol levels (LDL-C) through binding of the LDL receptor, resulting in the reduction of receptor recycling to the cell surface and the acceleration of receptor degradation in lysosomes (3). Both gain of function (GOF) and loss-of-function (LOF) mutations have been found in the PCSK9 gene (3). GOF mutations are linked to familial autosomal dominant hypercholesterolemia, a disease characterized by elevated plasma levels of LDL-C. In comparison, LOF mutations lead to low levels of LDL-C and protection against coronary heart disease.

  1. Seidah, N.G. et al. (2003) Proc. Natl. Acad. Sci. USA 100:928.
  2. Naureckiene, S. et al. (2003) Arch. Biochem. Biophys. 420:55.
  3. Costet, P. et al. (2008) Trends Biochem. Sci. 33:426.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Proprotein Convertase 9/PCSK9 Antibody (AF3888)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC, Neutralization, Western Blot.


Filter By Application
ICC
(1)
Neutralization
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(5)
All Species

Reviews for Proprotein Convertase 9/PCSK9 Antibody (AF3888) (5) 3.85

Average Rating: 3.8
(Based on 5 reviews)
We have 5 reviews tested in 1 species: Human.

Reviews using AF3888:
Filter by Applications
IF
(2)
IP
(2)
WB
(1)
All Applications
Filter by Species
Human
(5)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
IF Human 12/18/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedhPCKS9 in transfected HepG2 cells
SpeciesHuman

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFour-star review on pAbmAbs http://pabmabs.com/
  4
reviewed by:
Anonymous
IP Human 12/18/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedIP of human PCSK9
SpeciesHuman

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFour-star review on pAbmAbs http://pabmabs.com/
  3
reviewed by:
Anonymous
IP Human 12/18/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedRecombinant hPCSK9 expressed in 293 and HepG2 cells
SpeciesHuman

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsThree-star review on pAbmAbs http://pabmabs.com/
  4
reviewed by:
Anonymous
IF Human 12/18/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedCells transfected with hPCSK9 cDNA
SpeciesHuman

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFour-star review on pAbmAbs http://pabmabs.com/
  4
reviewed by:
Anonymous
WB Human 12/18/2014
View

Summary

ApplicationWestern Blot
Sample TestedRecombinant hPCSK9 expressed in 293 and HepG2 cells
SpeciesHuman

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFour-star review on pAbmAbs http://pabmabs.com/

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Proprotein Convertase 9/PCSK9 Antibody (AF3888) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF3888

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Bioinformatics Tool for Proprotein Convertase 9/PCSK9 Antibody (AF3888)

Discover related pathways, diseases and genes to Proprotein Convertase 9/PCSK9 Antibody (AF3888). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Proprotein Convertase 9/PCSK9 Antibody (AF3888)

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Recent Reviews

3.8
5
0
4
4
3
1
2
0
1
0

 
Anonymous
12/18/2014
Application: IF
Species: Human

 
Anonymous
12/18/2014
Application: IP
Species: Human

 
Anonymous
12/18/2014
Application: IP
Species: Human

Bioinformatics

Gene Symbol PCSK9
Uniprot