Proprotein Convertase 9/PCSK9 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3888) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pre-Proprotein Convertase 9/PCSK9 at approximately 80 kDa and mature Proprotein Convertase 9/PCSK9 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Recombinant Human Proprotein Convertase 9/PCSK9 protein was serially diluted 2-fold and captured by Rat Anti-Human Proprotein Convertase 9/PCSK9 Monoclonal Antibody (Catalog # MAB38883) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3888) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Proprotein Convertase 9/PCSK9 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human PCSK9 isoform 1
  Gln31-Gln692
  Accession # Q8NBP7
• Specificity: Detects human PCSK9 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse PCSK9 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PCSK9
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • ELISA
  • Immunoprecipitation 25 ug/mL
• Control:

  Proprotein Convertase 9/PCSK9 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Proprotein Convertase 9/PCSK9 Antibody [Unconjugated]

• EC 3.4.21
• EC 3.4.21.111
• FH3
• FH3neural apoptosis regulated convertase 1
• FHCL3
• HCHOLA3
• hypercholesterolemia, autosomal dominant 3
• LDLCQ1
• NARC1
• NARC-1
• NARC-1convertase subtilisin/kexin type 9 preproprotein
• NARC1EC 3.4.21.-
• Neural apoptosis-regulated convertase 1
• PC9
• PCSK9
• Proprotein Convertase 9
• proprotein convertase subtilisin/kexin type 9
• Subtilisin/kexin-like protease PC9

Background

The human PCSK9 gene encodes Proprotein Convertase 9 (PC9), which is also known as Neural Apoptosis Regulated Convertase 1 (NARC1) (1). The deduced amino acid sequence of human PCSK9 consists of a signal peptide (residues 1-30), a pro peptide (residue 31-152), and a mature chain (residues 153-692) that contains a serine protease domain (residues 161-431) found in members of the furin/PC family. PCSK9 protease activity may be limited, since it has only been demonstrated through its own autocatalytic processing (2). After the autocleavage in the ER, the pro domain and mature chain exit the cell together through non-covalent interactions (3). PCSK9 is a key regulator of LDL-cholesterol levels (LDL-C) through binding of the LDL receptor, resulting in the reduction of receptor recycling to the cell surface and the acceleration of receptor degradation in lysosomes (3). Both gain of function (GOF) and loss-of-function (LOF) mutations have been found in the PCSK9 gene (3). GOF mutations are linked to familial autosomal dominant hypercholesterolemia, a disease characterized by elevated plasma levels of LDL-C. In comparison, LOF mutations lead to low levels of LDL-C and protection against coronary heart disease.

1. Seidah, N.G. et al. (2003) Proc. Natl. Acad. Sci. USA 100:928.
2. Naureckiene, S. et al. (2003) Arch. Biochem. Biophys. 420:55.
3. Costet, P. et al. (2008) Trends Biochem. Sci. 33:426.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Proprotein Convertase 9/PCSK9 Antibody (AF3888)(5)

Publications using AF3888

• X Li, J Guo, N Liang, X Jiang, Y Song, S Ou, Y Hu, R Jiao, W Bai 6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells Front Pharmacol, 2018;9(0):159. 2018 [PMID: 29535632] (Western Blot, Human)
• C Gustafsen, D Olsen, J Vilstrup, S Lund, A Reinhardt, N Wellner, T Larsen, CBF Andersen, K Weyer, JP Li, PH Seeberger, S Thirup, P Madsen, S Glerup Heparan sulfate proteoglycans present PCSK9 to the LDL receptor Nat Commun, 2017;8(1):503. 2017 [PMID: 28894089] (ICC, Human)
• DeVay R, Yamamoto L, Shelton D, Liang H Common Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Epitopes Mediate Multiple Routes for Internalization and Function. PLoS ONE, 2015;10(4):e0125127. 2015 [PMID: 25905719] (Western Blot, Human)
• Tavori H, Giunzioni I, Linton M, Fazio S Loss of plasma proprotein convertase subtilisin/kexin 9 (PCSK9) after lipoprotein apheresis. Circ Res, 2013;113(12):1290-5. 2013 [PMID: 24122718] (Western Blot, Human)
• Duff CJ, Scott MJ, Kirby IT, Hutchinson SE, Martin SL, Hooper NM Antibody-mediated disruption of the interaction between PCSK9 and the low-density lipoprotein receptor. Biochem. J., 2009;419(3):577-84. 2009 [PMID: 19196236] (Neutralization, Human)

Reviews for Proprotein Convertase 9/PCSK9 Antibody (AF3888) (5)

Average Rating: 3.8

(Based on 5 reviews)

We have 5 reviews tested in 1 species: Human.

reviewed by: Anonymous IF Human 12/18/2014

Summary

• Application: Immunofluorescence
• Sample Tested: hPCKS9 in transfected HepG2 cells
• Species: Human

Blocking

• Blocking Details: n/a

Details

• Detection Notes: n/a

Comments

• Comments: Four-star review on pAbmAbs http://pabmabs.com/

reviewed by: Anonymous IP Human 12/18/2014

Summary

• Application: Immunoprecipitation
• Sample Tested: IP of human PCSK9
• Species: Human

Blocking

• Blocking Details: n/a

Details

• Detection Notes: n/a

Comments

• Comments: Four-star review on pAbmAbs http://pabmabs.com/

reviewed by: Anonymous IP Human 12/18/2014

Summary

• Application: Immunoprecipitation
• Sample Tested: Recombinant hPCSK9 expressed in 293 and HepG2 cells
• Species: Human

Blocking

• Blocking Details: n/a

Details

• Detection Notes: n/a

Comments

• Comments: Three-star review on pAbmAbs http://pabmabs.com/

reviewed by: Anonymous IF Human 12/18/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Cells transfected with hPCSK9 cDNA
• Species: Human

Blocking

• Blocking Details: n/a

Details

• Detection Notes: n/a

Comments

• Comments: Four-star review on pAbmAbs http://pabmabs.com/

reviewed by: Anonymous WB Human 12/18/2014

Summary

• Application: Western Blot
• Sample Tested: Recombinant hPCSK9 expressed in 293 and HepG2 cells
• Species: Human

Blocking

• Blocking Details: n/a

Details

• Detection Notes: n/a

Comments

• Comments: Four-star review on pAbmAbs http://pabmabs.com/

Bioinformatics

• Gene Symbol: PCSK9
• Uniprot:
  • Q8NBP7()