Proprotein Convertase 9/PCSK9 Antibody (499920R) [Unconjugated]

Recombinant Human Proprotein Convertase 9/PCSK9 protein was serially diluted 2-fold and captured by Rat Anti-Human Proprotein Convertase 9/PCSK9 Monoclonal Antibody (Catalog # MAB38883) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3888) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: ELISA
• Clone: 499920R
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Proprotein Convertase 9/PCSK9 Antibody (499920R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Proprotein Convertase 9/PCSK9
  Arg29-Gln692
  Accession # Q8NBP7
• Specificity: Detects human Proprotein Convertase 9/PCSK9 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Proprotein Convertase 9/PCSK9 Antibody (499920R) [Unconjugated]

• EC 3.4.21
• EC 3.4.21.111
• FH3
• FH3neural apoptosis regulated convertase 1
• FHCL3
• HCHOLA3
• hypercholesterolemia, autosomal dominant 3
• LDLCQ1
• NARC1
• NARC-1
• NARC-1convertase subtilisin/kexin type 9 preproprotein
• NARC1EC 3.4.21.-
• Neural apoptosis-regulated convertase 1
• PC9
• PCSK9
• Proprotein Convertase 9
• proprotein convertase subtilisin/kexin type 9
• Subtilisin/kexin-like protease PC9

Background

The human PCSK9 gene encodes Proprotein Convertase 9 (PC9), which is also known as Neural Apoptosis Regulated Convertase 1 (NARC1) (1). The deduced amino acid sequence of human PCSK9 consists of a signal peptide (aa 1 to 30), a propeptide (aa 31 to 152), and a mature chain (aa 153 to 692) that contains a serine protease domain (aa 161 to 431) found in members of the furin/PC family. PCSK9 protease activity may be limited, since it has only been demonstrated through its own autocatalytic processing (2). After the autocleavage in the ER, the pro domain and mature chain exit the cell together through non‑covalent interactions (3). PCSK9 is a key regulator of LDL-cholesterol levels (LDL-C) through binding of the LDL receptor, resulting in the reduction of receptor recycling to the cell surface and the acceleration of receptor degradation in lysosomes (3). Both gain of function (GOF) and loss-of-function (LOF) mutations have been found in the PCSK9 gene (3). GOF mutations are linked to familial autosomal dominant hypercholesterolemia, a disease characterized by elevated plasma levels of LDL-C. In comparison, LOF mutations lead to low levels of LDL-C and protection against coronary heart disease.

1. Seidah, N.G. et al. (2003) Proc. Natl. Acad. Sci. USA 100:928.
2. Naureckiene, S. et al. (2003) Arch. Biochem. Biophys. 420:55.
3. Costet, P. et al. (2008) Trends Biochem. Sci. 33:426.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8NBP7()