Proprotein Convertase 1/PCSK1 Antibody

Western Blot: PC1/3 Antibody [NB120-3532] - Analysis of mouse pituitary extract.

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Proprotein Convertase 1/PCSK1 Antibody Summary

• Description: Novus Biologicals Rabbit Proprotein Convertase 1/PCSK1 Antibody (NB120-3532) is a polyclonal antibody validated for use in WB. Anti-Proprotein Convertase 1/PCSK1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to residues S(111) V Q K D S A L D L F N D P M W N(127) of mouse PC1.
• Specificity: Prohormone Convertase 1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCSK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.4 ug/ml
• Application Notes: WB: Detects an approx. 80 kDa protein representing PC1 from mouse pituitary extract.

Reactivity Notes

Expected cross-reactivity with Bovine base on sequence identity.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Proprotein Convertase 1/PCSK1 Antibody

• BMIQ12;NEC1;Neuroendocrine convertase 1;PC1;PC1/3;PC3;SPC3
• NEC1
• PCSK1
• Proprotein Convertase 1

Background

The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PC1/3 Antibody (NB120-3532)(3)

Publications using NB120-3532

• Walters J, Walters C, Cameron B, George L Elongator regulates the melanocortin satiety pathway Biochemical and Biophysical Research Communications [PMID: 35537288] (WB, Mouse)
• Evans JF, Rodriguez S, Ragolia L. ACTH promotes chondrogenic nodule formation and induces transient elevations in intracellular calcium in rat bone marrow cell cultures via MC2-R signaling. Cell Tissue Res 2013-01-29 [PMID: 23358747] (WB, Rat)
• Evans JF, Fernando A, Ragolia. Functional melanocortin-2 receptors are expressed by mouse aorta-derived mesenchymal progenitor cells Mol Cell Endocrinol 2012-05-15 [PMID: 22306084] (WB, Mouse)

Bioinformatics

• Gene Symbol: PCSK1