Prominin 2 Antibody (244029) [Unconjugated]

Prominin 2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Prominin 2 Monoclonal Antibody (Catalog # MAB2024) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 244029
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Prominin 2 Antibody (244029) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Prominin 2
  Ala27-Leu834
  Accession # AAM10541
• Specificity: Detects human Prominin 2. Stains human Prominin 2-transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PROM2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prominin 2 Antibody (244029) [Unconjugated]

• hPROML2
• MGC138714
• PROM2
• PROM-2
• Prominin 2
• prominin-2
• Prominin-like protein 2
• prominin-related protein
• PROML2

Background

Prominin 2 is a pentaspan membrane glycoprotein predominantly expressed in neuroepithelial cells, hematopoietic stem cells and epithelial cells of the adult kidney and digestive tract (1, 2). Prominin 2 is a 112-kDa glycoprotein structurally related to prominin 1 (CD133). The amino acid identity between prominin 1 and prominin 2 is low (<30%), but they exhibit some redundant functions.

1. Fargeas, C.A. et al. (2003) J. Biol. Chem. 278(10):8586.
2. Fargeas, C.A. et al. (2003) Stem Cells 21(4):506.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prominin 2 Antibody (MAB2024)(1)

Publication using MAB2024

• Santelli A, Sun I, Eirin A, Abumoawad A, Woollard J, Lerman A, Textor S, Puranik A, Lerman L Senescent Kidney Cells in Hypertensive Patients Release Urinary Extracellular Vesicles. J Am Heart Assoc, 2019-06-01;8(11):e012584. 2019-06-01 [PMID: 31433703] (ICC, Human)

Bioinformatics

• Gene Symbol: PROM2
• Uniprot:
  • AAM10541()