Prolyl Oligopeptidase/PREP Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Prolyl Oligopeptidase/PREP Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PREP
  Leu2-Pro710
  Accession # P48147
• Specificity: Detects human PREP in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse PREP is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PREP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prolyl Oligopeptidase/PREP Antibody [Unconjugated]

• dJ355L5.1 (prolyl endopeptidase)
• EC 3.4.21.26
• MGC16060
• PE
• PEP
• Post-proline cleaving enzyme
• PREP
• prolyl endopeptidase
• Prolyl Oligopeptidase
• rPop

Background

Prolyl Oligopeptidase is a serine peptidase displaying specificity for the cleavage of Pro-Xaa bonds of oligopeptide substrates (1, 2). The peptidase is known to hydrolyze a variety of biologically active peptides such as bradykinin, substance P, neurotensin, and vasopressin (3). Because of its action on neuropeptides, Prolyl Oligopeptidase is considered to be involved in processes such as learning, memory, and depression (4).

1. Tarrago, T. et al. (2005) J. Pept. Sci. 11:283.
2. Yoshimoto, T. et al. (1977) Biochemistry. 16:2942.
3. Wilk, S. (1983) Life Sci. 33:2149.
4. Maes, M. et al. (1994) Biol. Psychiatry. 35:545.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Prolyl Oligopeptidase/PREP Antibody (AF4308)(3)

Publications using AF4308

• Kenneth W. Jackson, Victoria J. Christiansen, Vivek R. Yadav, Robert Silasi-Mansat, Florea Lupu, Vibhudutta Awasthi et al. Suppression of Tumor Growth in Mice by Rationally Designed Pseudopeptide Inhibitors of Fibroblast Activation Protein and Prolyl Oligopeptidase1 Neoplasia 1/23/2015 [PMID: 25622898]
• Savolainen M, Yan X, Myohanen T, Huttunen H Prolyl oligopeptidase enhances alpha-synuclein dimerization via direct protein-protein interaction. J Biol Chem, 2015-01-02;290(8):5117-26. 2015-01-02 [PMID: 25555914] (Western Blot, Mouse)
• Duan L, Ying G, Danzer B, Perez R, Shariat-Madar Z, Levenson V, Maki C The prolyl peptidases PRCP/PREP regulate IRS-1 stability critical for rapamycin-induced feedback activation of PI3K and AKT. J Biol Chem, 2014-06-16;289(31):21694-705. 2014-06-16 [PMID: 24936056] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PREP
• Uniprot:
  • P48147()