Recombinant Rat Prolactin R Protein

Product Discontinued

Recombinant Rat Prolactin R Protein Summary

• Description: Recombinant bioactive protein containing 206 amino acids for Rat Prolactin R
  
  

  Source:E. coli
  Amino Acid Sequence:(P05710 )

• Preparation Method: Determined to be >97% pure by SDS-PAGE
• Details of Functionality: Determined by the dose-dependent inhibition of Prolactin stimulated proliferation of Nb2 cells and by high affinity binding of ovine Prolactin and other lactogenic hormones.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: Prlr
• Purity: >97%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 24.12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: Sterile filtered and lyophilized from a 1 mg/ml solution with 0.0045 mM NaHCO3.
• Concentration: Lyoph
• Purity: >97%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in sterile dH2O to a concentration of 0.1 -1 mg/ml. This solution can then be diluted into other aqueous buffers.

Alternate Names for Recombinant Rat Prolactin R Protein

• delta 4-delta 7/11 truncated prolactin receptor
• delta 4-SF1b truncated prolactin receptor
• hPRL receptor
• hPRLrI
• PRLR
• PRL-R
• Prolactin R
• prolactin receptor delta 7/11
• prolactin receptor
• ProlactinR
• secreted prolactin binding protein

Background

Prolactin is a pituitary hormone that plays a role in the stimulation of milk production, salt and H2O regulation, growth, development and reproduction. The primary step in its action is the binding to a specific membrane receptor (prolactin receptor) which belongs to the superfamily of class 1 cytokine receptors. Prolactin is a hormone involved in a range of significant functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. Prolactin exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane Prolactin receptor. PRLR varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. Recombinant human Prolactin Receptor (Extra Cellular Domain) produced in E.Coli is a non-glycosylated, Polypeptide chain containing 206 amino acids and having a molecular mass of 24.12 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: Prlr