Prolactin R Antibody (T6)

Images

 
Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (T6) [NB300-531] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin ...read more
Immunohistochemistry-Paraffin: Prolactin R Antibody (T6) [NB300-531] - Normal biopsies of deparaffinized Rat pituitary gland tissue at a dilution of 1:50.
Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (T6) [NB300-531] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin ...read more
Immunocytochemistry/ Immunofluorescence: Prolactin R Antibody (T6) [NB300-531] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin ...read more
Immunohistochemistry: Prolactin R Antibody (T6) [NB300-531] - Staining of PRL receptor in NB2 cells using NB300-531.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, ICC, IF
Clone
T6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Prolactin R Antibody (T6) Summary

Immunogen
Purified rat liver PRL receptor.
Specificity
Detects prolactin (PRL) receptor in rat tissues. This does not cross-react with Growth Hormone (GH) receptor.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PRLR
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:25 - 1:200
  • Immunohistochemistry-Paraffin 1:25 - 1:200
  • Immunocytochemistry 5 ug/ml
  • Immunofluorescence 1:200
Application Notes
WB: Detects an approx. 42 kDa protein representing PRL receptor in NB2 cell lysate.
Publications
Read Publications using NB300-531.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
No buffer
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 0.1 ml PBS.

Alternate Names for Prolactin R Antibody (T6)

  • delta 4-delta 7/11 truncated prolactin receptor
  • delta 4-SF1b truncated prolactin receptor
  • hPRL receptor
  • hPRLrI
  • PRLR
  • PRL-R
  • Prolactin R
  • prolactin receptor delta 7/11
  • prolactin receptor
  • ProlactinR
  • secreted prolactin binding protein

Background

Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Prolactin R Antibody (NB300-531)(6)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Prolactin R Antibody (NB300-531) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-531AF405
Alexa Fluor 488 Labeled NB300-531AF488
Alexa Fluor 647 Labeled NB300-531AF647
Alexa Fluor 700 Labeled NB300-531AF700
DyLight 350 Labeled NB300-531UV
DyLight 405 Labeled NB300-531V
DyLight 488 Labeled NB300-531G
DyLight 680 Labeled NB300-531FR
DyLight 755 Labeled NB300-531IR

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Bioinformatics

Gene Symbol PRLR
Entrez
Uniprot